Carbohydrate Analysis Facility

我们实验室的研究涉及碳水化合物活性酶的结构和功能关系，催化涉及糖的生化反应的蛋白质。 这些研究涉及细菌、酵母和植物生理学，其核心是碳水化合物的分析。为此，申请人曾为圭尔夫大学及其他大学的研究人员维护和操作碳水化合物分析设施超过15年。 该设施的核心是Dionex高pH阴离子交换色谱仪，结合脉冲安培检测，用于解析和检测十亿分之一克以下的碳水化合物。 鉴于技术的进步，无论是在硬件还是软件方面，都需要一个全新的系统。 因此，我们建议购买Dionex ICS-5000离子色谱系统用于碳水化合物分析。 该系统采用CarboPac PA 200分析柱，配备脉冲电化学检测器（PED）和金电极，用于检测碳水化合物（以及氨基酸），以及AS-AP自动进样器，用于管理每个实验需要分析的许多样品。 该系统由Chrominance on 7软件控制。

Research in our laboratories concerns the structure and function relationship of carbohydrate-active enzymes, proteins that catalyse biochemical reactions involving sugars. Central to these investigations, involving bacterial, yeast, and plant physiology, is the need for the analysis of carbohydrates. To this end, the applicant used to maintain and operate a carbohydrate analysis facility for researchers at the University of Guelph and beyond for over 15 years. At the core of this facility was a Dionex high-pH anion-exchange chromatograph coupled with pulsed-amperometric detection for the resolution and detection carbohydrates at levels below one billionth of a gram. However, key elements of this system have failed and they need to be replaced. Given advances in technology, both in hardware and software, a completely new system is required. Thus, we propose to purchase a Dionex ICS-5000 Ion Chromatography System for Carbohydrate Analysis. This system utilising a CarboPac PA200 Analytical Column would be equipped with a pulsed electrochemical detector (PED) and gold electrode for detection of carbohydrates (and amino acids too) and an AS-AP Autosampler to manage the many samples requiring analysis for each experiment. The system is controlled by the Chromeleon 7 software.