"The protein interaction network of Cdt1, an essential replication protein"

为了使细胞生长和分裂，它必须复制其遗传内容。在植物和动物等细胞中，许多调节遗传物质复制的因素与在用于烘焙面包或酿造啤酒的酵母中发现的相同。Davey实验室使用酵母中可用的优秀遗传和生化工具来询问有关细胞分裂控制和遗传物质复制的基本问题。在这项提案中，他们将研究控制遗传物质何时被复制所需的一种必需蛋白质的相互作用。它们将确定发生了什么相互作用，相互作用需要蛋白质的哪些部分以及这些相互作用如何影响细胞中的活动。总之，这些研究将揭示有助于控制细胞生长和分裂中这一关键步骤的相互作用网络。

In order for a cell to grow and divide, it must copy its genetic content. In cells such as plant and animals, many of the factors that regulate the copying of genetic material are the same as are found in yeast used in baking bread or brewing beer. The Davey lab uses the excellent genetic and biochemical tools available in yeast to ask fundamental questions about the control of cell division and the copying of genetic material. In this proposal, they will examine the interactions of an essential protein required for to control when the genetic material will be copied. They will determine what interactions occur, what parts of the protein are required for the interaction and how these interactions affect activities in the cell. Together, these studies will reveal the interaction network that helps control this key step in the growth and division of cells.