Growth and development of the wheat starch granule associated proteome during growth and development of the seed

不断增长的人口依赖谷物作为其主要食物来源。对小麦、水稻和玉米等谷物的成分进行了生化和营养分析，以提高其数量和质量。谷类作物农艺学的生长和发展必须辅之以多层面的谷粒分析：了解营养决定因素，增强分子机制以提高对病原体和害虫的抵抗力，并使我们能够更好地设计淀粉涂层，麦芽制造和酿造以及生物燃料等工业流程。这个谷物蛋白质组学项目以尽可能高的分辨率水平，蛋白质质谱法，检查了站立作物中谷物发育的七到八周。最快的方法来剖析遗传和代谢调控网络的粮食发展，特别是淀粉的生物合成是通过识别所有潜在的蛋白质组分在颗粒表面。该计划结合了植物生物学，系统生物学和亚细胞显微镜的元素。这些一系列的调查覆盖了广泛的蛋白质组学方法，使用高通量质谱与液相色谱相结合。因此，我们提出了具体的研究，旨在提高我们的理解颗粒如何增长内生长胚乳细胞。该计划也将成为植物育种家和食品生物技术专家的重要资源，因为发现的蛋白质可以用于非食品和食品行业的改良作物设计。

The growing human population relies on cereal grains for its major food source. The composition of grains like wheat, rice and maize have been analyzed biochemically and nutritionally to enhance their quantity and quality. Growth and development of the agronomy of cereal crops must be complemented by analysis of the grain kernels at many levels: understand the nutritional determinants, enhance molecular mechanisms to improve resistance to pathogens and pests, and allow us to better engineer industrial processes like starch coatings, malting and brewing and biofuels. This cereal proteomics program examines the seven to eight weeks of grain development in the standing crop at the highest possible level of resolution, protein mass spectrometry. The fastest way to dissect the genetic and metabolic regulatory networks governing grain development and in particular starch biosynthesis is by identifying all potential protein components at the granule surface. This program combines elements of plant biology, systems biology and subcellular microscopy. These series of investigations are overlayed with a broad scope proteomics approach using high-throughput mass spectrometry combined with liquid chromatography. Therefore, we propose specific research aims to improve our understanding of how granules grow inside growing endosperm cells. The program will also be an important resource for plant breeders and food biotechnologists because the discovered proteins can be deployed in the design of improved crops in both the non-food and food industries.