Freeze Dryer for extraction of high value biomolecules

冷冻干燥机(Labcono Freezone Plus 2.5 L级联)非常有效地干燥、浓缩和蒸发各种样品(牛奶、尿素、抗癌药物、抗生素、营养食品、鱼类样品、植物样品、真菌和细菌)、各种溶剂的蒸发、肽的提取、各种聚合物和纳米颗粒的干燥。所要求的冷冻干燥机对加利博士、布鲁克斯博士、巴奇博士、加尼姆博士、吉尔博士和特鲁斯特鲁普·汉森博士的研究小组至关重要。Al Taweel博士、Mazzanti博士和Yildiz博士也将使用它。这些小组多年来用来准备精矿以进行分级和生物分析实验的现有设备是一台二手(已有20年历史)的过时冷冻干燥机。这台老式冷冻干燥机上的泵也是二手的(有30年的历史)，因此需要经常保养和昂贵的维修和维护。产生的真空不足以满足我们的干燥要求。因此，拟议的设备对于目前和未来的以下研究项目至关重要：干燥蓝莓、Haskap浆果、鱼和鱼类加工废物样本、各种纳米颗粒、细菌和真菌以及各种营养食品(鱼油、omega-3脂肪酸、蛋白质、氨基酸)和药品的分离和提取。(生物活性多肽(T2D)、杰霉素、糜蛋白酶、胃酶、胶原酶、酶抑制剂：α-1抗蛋白酶、α-2巨球蛋白、抗凝剂：肝素和类肝素糖胺聚糖)。在没有冷冻干燥机的情况下，不可能从鱼血中的其他脂蛋白(用于测定特定脂肪酸的稳定碳同位素)中分离出乳杆菌微粒。需要所需的冷冻干燥器，以便从细菌细胞中分离/提取和提纯由天然生物聚合物壳聚糖和抗生素(杰多霉素)形成的纳米颗粒。该设备将用于培训52名HQP(7名本科生、33名研究生、2名PDF和10名技术人员)。

The Freeze Dryer (Labconco Freezone Plus 2.5 L Cascade) is very effective in drying, concentration and evaporation of various samples (milk, urea, anti-cancer drug, antibiotics, nutratceuticals, fish samples, plant samples, fungi and bacteria), evaporation of various solvents, peptide extraction, drying of various polymers and nanoparticles. The requested Freeze Dryer is crucial for the research groups of Dr. Ghaly, Dr. Brooks, Dr. Budge, Dr. Ghanem, Dr. Gill and Dr. Truelstrup Hansen. It will be also used by Dr. Al Taweel, Dr. Mazzanti and Dr. Yildiz. The currently available unit on which the groups relied upon to prepare concentrates for experiments on fractionation and bio-assays for years is a second-hand (20 years old) obsolete freeze dryer. The pump on this old freeze dryer is also second hand (30 years old) and as such requires constant attention and expensive repair and maintenance. The vacuum produced is inadequate for our drying requirements. Thus, the proposed equipment is essential for the current and future research projects on drying blueberries, haskap berries, fish and fish processing waste samples, various nanoparticles, bacteria and fungi and separation and extraction of various nutraceuticals (fish oil, omega-3 fatty acids, proteins, aminoacids) and pharmaceuticals. (bioactive peptides (T2D), jadomycin, chymotrypsin, pepsin, collagenase, enzyme inhibitors: alpha-1 antiproteinase, alpha-2 macroglobulin, anticoagulants: heparin and heparin-like glycosaminoglycans). It is impossible to carry out the separation of chylomicrons from the other lipoproteins in fish blood (for measuring the stable carbon isotopes of specific fatty acids) without freeze dryer. The requested freeze dryer is needed in order to separate/extract and purify the nanoparticles formulated from the natural biopolymer chitosan and antibiotics (jadomycin) from bacteria cells. This equipment will be utilized for training of 52 HQP (7 undergraduate students, 33 graduate students, 2 PDF and 10 technicians).