Molecular architecture in gene expression and virus-host interactions

我研究的目的是了解蛋白质如何组装成适合特定功能的结构。通过使用核磁共振波谱和x射线晶体学等技术，可以实现可视化蛋白质组合差异所需的原子级细节。我的提案中的三个目标涵盖了细胞信号传导的三个一般领域。第一个目的是研究一对Sox9蛋白协同工作如何激活细胞中不同于单独工作的Sox9蛋白的发育程序。第二个目的是研究细菌转录因子及其启动子之间相互作用的性质，这些启动子驱动抗生素耐药性。第三个目标是研究在病毒感染过程中，一种名为ea8.5的蛋白质是如何改变细菌遗传程序的。本提案中描述的基础研究将用于了解细胞信号传导的新方面，并帮助设计针对疾病的选择性疗法。

The aim of my research is to understand how proteins assemble into an architecture that is well suited to a specific function. The atomic level of detail required to visualize differences in protein assemblies is achieved by using techniques such as NMR spectroscopy and X-ray crystallography that are available locally to my laboratory. The three aims in my proposal cover three general areas of cell signaling. The first aim examines how a pair of Sox9 proteins working together is capable of activating a different developmental program in a cell than one Sox9 protein working alone. The second aim examines the nature of the interactions between a bacterial transcription factor and its promoters that drive antibiotic resistance. The third aim examines how a bacterial genetic program is altered during viral infection by a protein called ea8.5. The basic research described in this proposal will be used to understand the new aspects of cell signaling and help design selective therapies against disease.