Moulding fluorescent proteins into biotools: engineering topology, surfaces and chromophores

蛋白质是我们所知的生命所必需的惊人的分子机器。经过世纪的蛋白质研究，我们现在对蛋白质如何能够执行其大量功能有了很好的了解。一个非常有用的蛋白质家族，我们现在已经非常了解了，是固有荧光蛋白（FP）家族，包括来自水母的绿色FP和来自珊瑚的各种颜色的许多同系物。FP具有独特的（在蛋白质中）在其自身的三维结构内产生可见波长荧光团的能力，因此可以吸收可见光并将其重新发射为红移色调。在过去的十年中，这些非凡的蛋白质工具使生物研究取得了许多令人兴奋的进展，因为将FP基因插入活细胞或组织中可以让人们“看到”否则无法检测到的生物结构。FP被认为引发了活细胞荧光显微镜普及的爆炸性和持续增长;最终将2008年诺贝尔化学奖授予FP研究的先驱。

Proteins are amazing molecular machines that are essential for Life as we know it. After a century of protein research, we now have a very good understanding of how proteins are able to carry out their vast array of functions. One extraordinarily useful family of proteins, which we now understand quite well, is the family of intrinsically fluorescent proteins (FPs) that includes the green FP from Aequorea jellyfish and its numerous homologues of various color from coral. FPs have the unique (among proteins) ability to generate a visible wavelength fluorophore within their own 3-dimensional structure and thus can absorb visible light and reemit it as a red-shifted hue. In the past decade, these remarkable protein tools have enabled many exciting advances in biological research because genetically inserting a FP into a living cell or tissue allows one to 'see' biological structures that would otherwise be undetectable. FPs are credited with sparking the explosive and continuing growth in the popularity of live cell fluorescence microscopy; culminating in the awarding of 2008 Nobel Prize in Chemistry to the pioneers of FP research.