Assessing the immunomodulatory properties of yeast using primary intestinal epithelial cells and bovine macrophages
Assessing the immunomodulatory properties of yeast using primary intestinal epithelial cells and bovine macrophages
批准号:
462113-2014
负责人:
Karrow, Niel
金额:
$0.83万
依托单位:
依托单位国家:
加拿大
项目类别:
Engage Plus Grants Program
财政年份:
2014
资助国家:
加拿大
项目状态:
已结题
起止时间:
2014-01-01 至 2015-12-31
中文摘要
由于酵母益生菌及其细胞壁成分(CWCs)已被用于治疗包括人类在内的不同物种的肠道炎症性疾病,它们可能有助于预防约翰氏病(一种由鸟分枝杆菌副结核(MAP)引起的反刍动物慢性炎症性肠病)。在我们之前的研究中,我们开发了体外试验,使我们能够利用MAP作为靶病原体对牛乳腺上皮细胞(Mac-T)评估特定酵母cwc的抗粘附特性。此外,用牛巨噬细胞系(BoMac)来评估酵母CWC潜在的免疫调节特性。拟议的研究将继续这项研究,并着重于潜力
英文摘要
Since yeast probiotics and their cell wall components (CWCs) are being used to treat enteric inflammatory diseases in different species including humans, they may be useful for preventing Johne's disease, a chronic inflammatory bowel disease of ruminants caused by Mycobacterium avium spp. paratuberculosis (MAP). In our previous study, we developed in vitro assays that allowed us to assess the anti-adhesive properties of specific yeast CWCs using MAP as a target pathogen on bovine mammary epithelial cells (Mac-T). Additionally, a bovine macrophage cell line (BoMac) was used to assess potential yeast CWC immunomodulatory properties. The proposed study will follow-up this research and focus on the potential
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