Genetic markers and Bovine Johne's disease

遗传标记和牛约翰氏病

• 批准号: 496214-2016
• 负责人: Karrow, Niel
• 金额: $ 2.7万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=655147
• 关键词: Genetic; markers; Bovine; Johne; disease

Johne's disease (JD) is a disease with great economic implications to the dairy industry and is also attracting**significant public health concern owing to the possible zoonotic nature of its causative bacteria,**Mycobacterium avium subsp. paratuberculosis. The lack of availability of vaccines with high efficacy as well**as effective treatments has hindered control of JD worldwide. Given its heritable nature, as indicated in various**studies, one means of reducing the incidence of JD is by employing genetic selection in breeding programs to**produce offspring with enhanced disease resistance. To achieve this, it is necessary to identify genetic markers**associated with JD and to assess their biological relevance. Over the past decade, a number of genomic studies**have identified different genetic markers associated with JD. Inconsistent findings from these studies suggest**that JD is a complex disease that is polygenic in nature with many potential genetic markers influencing disease**resistance. There is a need to further validate these genetic markers in independent populations before they can**be used in genetic selection programs, and this is the aim of the present study. The specific objectives are to: a)**validate and confirm previous associations with genetic markers and JD in a Canadian Holstein population, b)**assess potential impact of these markers on production and health traits, and c) characterize their biological**relevance in vitro using genetically modified bovine cell lines. New knowledge obtained from this study will**help dairy industry move forward to enhance resistance to JD through genetic selection.

约翰氏病(JD)是一种对奶牛业具有重大经济影响的疾病，由于其致病细菌可能具有人畜共患病性质，也引起了**重大的公共卫生关注**禽型分枝杆菌亚种。副结核。缺乏高效的疫苗以及有效的治疗方法**阻碍了世界范围内对JD的控制。正如各种研究表明的那样，鉴于其可遗传的性质，减少JD发病率的一种方法是在育种计划中使用遗传选择，以**产生具有增强抗病能力的后代。为了实现这一点，有必要确定与JD相关的遗传标记**并评估它们的生物学相关性。在过去的十年里，许多基因组研究**已经确定了与JD相关的不同遗传标记。这些研究不一致的发现表明**JD是一种复杂的疾病，本质上是多基因的，有许多潜在的遗传标记影响疾病**的抵抗力。有必要在独立群体中进一步验证这些遗传标记，然后才能**将它们用于遗传选择程序，这就是本研究的目的。具体目标是：a)**在加拿大荷斯坦牛群体中验证和确认先前与遗传标记和JD的关联，b)**评估这些标记对生产和健康性状的潜在影响，以及c)使用转基因牛细胞系在体外表征它们的生物学**相关性。本研究获得的新知识将有助于奶业通过遗传选择提高对JD的抗性。