Genetic markers and Bovine Johne's disease

遗传标记和牛约翰氏病

• 批准号: 496214-2016
• 负责人: Karrow, Niel
• 金额: $ 2.7万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=655147
• 关键词: Genetic; markers; Bovine; Johne; disease

Johne's disease (JD) is a disease with great economic implications to the dairy industry and is also attracting**significant public health concern owing to the possible zoonotic nature of its causative bacteria,**Mycobacterium avium subsp. paratuberculosis. The lack of availability of vaccines with high efficacy as well**as effective treatments has hindered control of JD worldwide. Given its heritable nature, as indicated in various**studies, one means of reducing the incidence of JD is by employing genetic selection in breeding programs to**produce offspring with enhanced disease resistance. To achieve this, it is necessary to identify genetic markers**associated with JD and to assess their biological relevance. Over the past decade, a number of genomic studies**have identified different genetic markers associated with JD. Inconsistent findings from these studies suggest**that JD is a complex disease that is polygenic in nature with many potential genetic markers influencing disease**resistance. There is a need to further validate these genetic markers in independent populations before they can**be used in genetic selection programs, and this is the aim of the present study. The specific objectives are to: a)**validate and confirm previous associations with genetic markers and JD in a Canadian Holstein population, b)**assess potential impact of these markers on production and health traits, and c) characterize their biological**relevance in vitro using genetically modified bovine cell lines. New knowledge obtained from this study will**help dairy industry move forward to enhance resistance to JD through genetic selection.

约翰氏病 (JD) 是一种对乳​​制品行业具有重大经济影响的疾病，并且由于其致病细菌**鸟分枝杆菌亚种可能具有人畜共患的性质，因此也引起了**重大的公共卫生关注。副结核病。由于缺乏高效疫苗和有效治疗方法，阻碍了全世界对 JD 的控制。鉴于其遗传性质，正如各种研究表明的那样，减少 JD 发病率的一种方法是在育种计划中采用遗传选择，以产生具有增强抗病性的后代。为了实现这一目标，有必要识别与 JD 相关的遗传标记**并评估其生物学相关性。在过去的十年中，许多基因组研究**已经确定了与 JD 相关的不同遗传标记。这些研究的不一致结果表明**JD 是一种复杂的多基因疾病，具有许多影响疾病**抵抗力的潜在遗传标记。在将这些遗传标记用于遗传选择计划之前，需要在独立群体中进一步验证这些遗传标记，这就是本研究的目的。具体目标是：a)**验证并确认加拿大荷斯坦群体中遗传标记和 JD 的先前关联，b)**评估这些标记对生产和健康性状的潜在影响，以及 c) 使用转基因牛细胞系在体外表征其生物**相关性。从这项研究中获得的新知识将**帮助乳制品行业通过基因选择增强对 JD 的抵抗力。