Refrigerated incubator shaker and centrifuge for cell culture

要求拨款购买冷冻培养摇床和冷冻离心机，这是用于细胞培养、细胞收获和过度生产和纯化重组蛋白的常用设备。冷藏培养箱将用于培养细菌细胞，并在低温下过量生产重组蛋白，这可以提高正确折叠的产量，用于表征的可溶性蛋白。冷冻离心机，用于收获细胞和分离细胞匀浆中不溶性碎片的可溶性蛋白质，将取代不再正常工作的离心机。这种设备是现代生物化学实验室中使用最广泛的设备之一，不仅是我们研究的重要组成部分，也是培养高素质人才的重要组成部分。该设备将用于研究植物细胞外基质（Suh）生物合成途径的进化，并表征微生物对外部刺激（Dahms）的反应。从Suh的研究中产生的知识应该证明在操纵非天然生物聚合物方面是有用的，可能导致农业（作物/森林控制）、生物技术（可生物降解聚合物）和医学（药物载体）方面的新应用。Dahms的研究具有相当大的潜力，可以通过确定持续接触低水平农药、抗真菌药物和金属对细胞的生理影响，为环境和人类健康带来益处。研究对人体细胞、病原体和病原体-宿主相互作用的影响将最终揭示微生物毒力、疾病易感性和进展。

Funds are requested for a refrigerated incubator-shaker and a refrigerated centrifuge, common equipment used for cell culture, cell harvesting and to overproduce and purify recombinant proteins. The refrigerated incubator will be used to culture bacterial cells and to overproduce recombinant protein at low temperature, which can lead to higher yields of correctly folded, soluble protein for characterization. The refrigerated centrifuge, used to harvest cells and to separate soluble proteins from insoluble debris in cell homogenates, will replace centrifuge that is no longer functional on a regular basis. Such equipment is among the most widely used in modern biochemistry laboratories, and is not only a vital part of our research but also an integral component for training highly qualified personnel. The equipment will be used to study the evolution of biosynthetic pathways for plant extracellular matrices (Suh) and characterize the response of microbes to external stimuli (Dahms). The knowledge generated from Suh's research should prove useful in manipulating unnatural biopolymers, possibly leading to novel applications in agriculture (crop/forest control), biotechhnology (biodegradable polymer) and medicine (drug carrier). Dahms' research has considerable potential to yield benefits to the environment and human health by determining the physiological effects to cells in response to persistent low-level pesticide exposure, antifungal drugs and metals. Studying the effects on human cells, pathogens and pathogen-host interactions will ultimately shed light on microbial virulence, disease susceptibility and progression.