Microscopy suite for time-lapse multi-wavelength live cell imaging

该申请用于资助购买一套仪器，将分子显微注射到活细胞中，并在温度受控的环境中对随后的过程进行成像。目前在Roger Guindon Hall还没有配备自动微量注射系统的专用成像平台。缺乏这样的基础设施大大限制了几个实验室的进展，有兴趣解决有关亚细胞组织和贩运的基本问题，如核仁结构，内体和自噬体贩运，线粒体组织和其他细胞过程。考虑到这些过程和架构的短暂或短暂性质，配备自动微量注射器的活细胞成像系统的缺乏严重限制了Roger Guindon Hall研究人员利用许多创新工具的能力，例如细胞过程，肽和干扰抗体的先进荧光传感器。该设备是所有现代生物学研究部门的特色，将使渥太华大学的许多研究人员能够在生命科学领域取得基础性发现。在帮助学院的几个研究实验室的同时，这个平台还将扩大我们实验室监督的众多博士后研究员，研究生和本科生所掌握的技术。

This application is for funding the purchase of a suite of instruments to microinject molecules into living cells and image the ensuing processes in a temperature controlled environment. There is currently no dedicated imaging platform equipped with an automated micro-injection system in Roger Guindon Hall. The lack of such infrastructure considerably limits the progress of several laboratories interested in addressing basic questions regarding subcellular organization and trafficking, such as nucleolar architecture, endosome and autophagosome trafficking, mitochondrial organization and other cellular processes. Given the transient or short-lived nature of these processes and architectures, the absence of a live-cell imaging system equipped with an automated micro-injector severely limits the ability of researchers in Roger Guindon Hall to utilize many innovative tools such as advanced fluorescent sensors of cellular processes, peptides, and interfering antibodies for example. This equipment is a feature of all modern biology research departments and will allow many researchers at the University of Ottawa to progress towards making fundamental discoveries in the life sciences. While helping several research laboratories in the Faculty, this platform will also expand the repertoire of techniques mastered by the numerous post-doctoral fellows, graduate and undergraduate students supervised in our laboratories.