Next generation sequencer for the genetic analysis of pathogens affecting bee species

国家蜜蜂诊断中心-TAC(技术接入中心)是加拿大唯一专门致力于蜜蜂物种的诊断、培训和应用研究的最先进的实验室。我们为养蜂业提供了一系列全面的分析，以确定蜜蜂种群的健康状况，其结果可用于管理目的。此外，在养蜂人的积极参与下，NBDC-TAC开展了以蜜蜂病理学为重点的应用研究。我们还与联邦、省、大学和学院的研究人员合作，研究野生蜜蜂物种的群体损失、疾病动态和病原体。近年来，下一代测序(NGS)已经改变了实验室进行基因分析和诊断的方式。我们现在能够在一次运行中在单个样本中识别几个目标。对影响生物体的已知疾病进行广泛而深入的筛查是可能的，甚至可以识别新的疾病。NBDC-TAC计划使用NGS技术来跟踪、维护和增加我们用于诊断的控制和结构；确定疾病株，识别新的致病生物体，并检测单核苷酸多态(SNPs)，其应用于筛选非洲化杂交对加拿大蜜蜂种群的国际贸易极其重要。这种基因分析与病原体量化和组织切片定位相结合，将使我们对疾病、症状和暴发有更完整、更清晰的了解。该项目将提高内部能力和绩效，通过挖掘以前无法获得的遗传信息，改变我们所有应用研究的范围。该设备完美地补充了NBDC-TAC的服务和研究，对于北艾伯塔省的一家教学机构来说是独一无二的。将对和平国家和其他地区的学生进行专门实验室技能培训，以帮助培养高素质人才。此外，我们将能够与AAFC、大学和其他国家和国际组织进行新的令人兴奋的应用研究合作。

The National Bee Diagnostic Centre - TAC (Technology Access Centre) is the only state-of-the-art laboratory in Canada dedicated exclusively to diagnostics, training and applied research for bee species. We provide a comprehensive array of analyses for the beekeeping industry to determine the health of bee stock, whose results can be used for management purposes. In addition, the NBDC-TAC with the active participation of beekeepers performs applied research focused on bee pathologies. We also collaborate with Federal, Provincial, University and College researchers on studies of colony losses, disease dynamics and pathogens of wild bee species. Next Generation Sequencing (NGS) has changed, in recent years, the way laboratories perform genetic analyses and diagnostics. We are now able to identify several targets in a single sample within a single run. It is possible to perform a wide and deep screening of known diseases affecting an organism, and even identify new ones. The NBDC-TAC plans to employ NGS technology to track, maintain and increase our controls and constructs used for diagnostics; determine disease strains, identify new disease-producing organisms, and detect Single Nucleotide Polymorphism (SNPs) whose application to screen Africanised hybrids is extremely important for Canada international trade of honey bee stock. This genetic analysis in combination with pathogen quantification and localization on tissue sections will give us a more complete and clearer picture of diseases, symptoms and outbreaks. The project will improve in-house capability and performance transforming the scope for all our applied research by digging into genetic information not available before. The equipment perfectly complements NBDC-TAC services and research, being unique for a teaching institute in Northern Alberta. Students in the Peace Country and from other regions will be trained on specialized laboratory skills contributing to the formation of highly qualified personnel. In addition, we will be able to enter into new exciting applied research collaborations with AAFC, Universities and other national and international organizations.