Development of an innovative bioelectrochemical process for the degradation of petroleum hydrocarbon contaminated soils/water : b) Enzymatic mode

本研究项目是基于学院-大学-产业合作的。它旨在满足TechnoRem Inc.的需求，TechnoRem Inc.是一家专门从事地下水和土壤处理的公司。如今，被石油烃污染的土壤和地下水是科学家面临的一大挑战。这个研发项目的目标是利用CTE(Gherrou，Daghrir&Huot)研究人员的专业知识和SK教授的专业知识。来自INRS-ETE的BRAR开发了一种创新的方法，该方法使用生物电化学过程，涉及酶反应和电化学氧化技术的协同作用，以降解污染土壤和地下水的复杂碳氢链(多环芳烃、C10-C50等)。我们的跨机构团队(INRS-CTE)提供的研发活动可以分为三个主要活动。第一部分包括确定合适的阳极和阴极，从而优化其碳氢化合物的氧化能力。碳氢化合物电化学氧化能力的优化将在CTE的实验室规模上进行。第二项任务将是确定和开发高效酶的纳米配方，并在INRS具有氧化降解碳氢链的能力。将在不同的实验条件下(pH、温度等)研究酶过程的效率。这项工作的第三部分将侧重于开发和应用一种用于降解污染土壤和地下水的碳氢化合物的混合生物电化学工艺(CTE-INRS)。在每个工艺(酶和电化学)的应用过程中确定的最佳工艺条件将被预先应用。该项目将受益于结合了电化学氧化技术和酶工艺的独特专业知识，以及处于领先地位的研发运营基础设施；这两个参数对于及时实现项目目标至关重要。

This research project is based on a college-university-industry collaboration. It aims to meet the needs of TechnoRem Inc., a company specializing in the treatment of groundwater and soils. Nowadays, soil and groundwater contaminated with petroleum hydrocarbons is a major challenge facing scientists. The objective of this R & D project is to leverage the expertise of researchers from CTE (Gherrou, Daghrir & Huot) and the expertise of Professor SK. Brar from INRS-ETE to develop an innovative method using bioelectrochemical process involving synergy of enzymatic reactions and electrochemical oxidation techniques for the degradation of complex hydrocarbon chains (PAHs, C10-C50, etc) that contaminate soil and groundwater. R & D activities offered by our inter-institutional team (INRS-CTE) can be divided into three main activities. The first part comprises identification of the anode and cathode that is appropriate and hence optimize their oxidation capacity of hydrocarbons. An optimization of electrochemical oxidation capacity of hydrocarbons will be performed at laboratory scale at CTE. The second task will be to identify and develop nano-formulations of efficient enzymes and having a capacity for oxidative degradation of hydrocarbon chains at INRS. The efficiency of the enzymatic process will be studied under different experimental conditions (pH, temperature, etc.). The third part of this work will be focused on the development and application of a hybrid bioelectrochemical process for the degradation of hydrocarbons that contaminate soil and groundwater (CTE-INRS). The optimum processing conditions determined during the application of each process (enzymatic and electrochemical) will be applied a priori. This project will benefit from a unique expertise combining electrochemical oxidation techniques and enzymatic processes, and an R & D operational infrastructure at the forefront; two parameters essential for the achievement of the project objectives in time.