Application of molecular techniques for rapid identification and characterization of rhizobacterial strains for enhanced crop performance

植物生长促进细菌是XiteBio Technologies业务的基础。这些细菌 配制成接种剂应用于农作物。这导致增强的生长和耐受性 压力，并可能减少对化肥和农药的应用需求。公司 希望扩大接种剂的生产线它目前有2,000个来自根际土壤的新菌株， 收集，其中一些菌株可能是接种剂开发的良好候选者。作为第一步， 需要确定这些分离物的分类身份，这将通过16 S rRNA 测序，以及使用BIOSTRUCTURE微生物ID系统。Illumina MiSeq仪器将在 适用于这种DNA序列分析。一旦知道了这些菌株的身份， 从进一步的分析中移除，将进行一系列植物生长促进试验。在此之后 分析，该公司应该有一个孕育剂产品的进一步发展系列潜在的菌株。

Plant growth promoting bacteria form the basis of XiteBio Technologies business. These bacteria are formulated into inoculants that are applied to crop plants. This results in enhanced growth and tolerance of stress, and potentially reduces the requirements for application of fertilizers and pesticides. The company wishes to expand its line of inoculants. It currently has 2,000 novel isolates from rhizosphere soil in its collection, and some of these strains might be good candidates for inoculant development. As a first step, it is necessary to determine the taxonomic identities of these isolates, and this will be done by 16S rRNA sequencing, as well as using the BIOLOG Microbial ID system. The Illumina MiSeq instrument will be adapted for this DNA sequence analysis. Once the identities are known, and potentially pathogenic strains are removed from further analysis, a series of plant growth promotion tests will be performed. Following this analysis, the company should have a series of potential strains for further development of inoculant products.