The protein-protein interaction network of Salmonella enterica, Campylobacter jejuni and Clostridium perfringens

随着公众要求从加拿大家禽业中淘汰抗生素生长促进剂（AGP）的压力越来越大，迫切需要负担得起且可广泛实施的替代品，以最大限度地降低肠道病原体（如产气荚膜梭菌）以及食品安全问题（如空肠弯曲杆菌和肠道沙门氏菌）的感染风险。为此，AbCelex Technologies Inc.率先使用基于抗体的饲料添加剂（纳米抗体），旨在减少家禽生产中的人畜共患病病原体。这项技术的一个关键挑战是鉴定代表纳米抗体开发的顺从目标的细菌蛋白质。一个重要的标准是确保纳米抗体具有高亲和力，但广泛的 在许多不同的被靶向的病原体菌株之间的交叉反应性。在这里，我们的目标是整合最近生成的物理膜蛋白复合物的地图，来自土方大肠杆菌，连同基因组序列数据的各种菌株的S。enterica、C. jejuni和C.产气荚膜杆菌，以鉴定那些保守性良好的组分以及它们在毒力途径中的作用。这些组件代表了纳米抗体开发的下一代目标，并将通过AbCelex的授权专有平台以及与Terebiznik博士的合作进行验证。最终，这项研究将有助于为新型纳米抗体抗菌剂奠定基础，这将有助于控制加拿大家禽业的病原体负担。

With increasing public pressure to eliminate antibiotic growth promoters (AGPs) from the Canadian poultry industry, affordable and broadly implementable alternatives that minimize risk of infection from enteric pathogens such as Clostridium perfringens, as well as those of food safety concern (e.g. Campylobacter jejuni and Salmonella enterica) are urgently needed. To this end, AbCelex Technologies Inc. are pioneering the use of antibody based feed additives (nanobodies) that target the reduction of zoonotic pathogens in poultry production. A key challenge in this technology is identifying bacterial proteins that represent amenable targets for nanobody development. A significant criteria is ensuring that nanobodies have high affinity but broad cross-reactivity across many different strains of the pathogens being targeted. Here our aim is to integrate recently generated maps of physical membrane protein complexes, derived for Eschericia coli, together with genome sequence data for a wide variety of strains of S. enterica, C. jejuni and C. perfringens, to identify those components that are well conserved as well as their role in virulence pathways. Such components represent the next generation of targets for nanobody development and will be validated through exisiting proprietary platform at AbCelex as well as a collaboration with Dr Terebiznik. Ultimately this research will help lay the foundation for novel nanobody antimicrobials that will help control pathogen burden across the poultry sector in Canada.