Development of in vitro propagation method of commercially valuable woody plants for indoor usage

苗圃技术不仅在粮食生产方面得到了大力发展，而且在园艺和室内植物方面也得到了发展。随着家庭、办公室和公共场所对室内植物的高需求，植物苗圃业务正在蓬勃发展。目前，加拿大人正在从世界各地进口许多不同的植物品种，并正在繁殖它们以满足不断增长的需求。根据加拿大统计局的数据，2013年加拿大温室产业共生产了14，700，000株紫云英和卡隆（两种主要的室内开花植物）。引进一种新的植物，将在同一个市场上竞争的菊花和卡勒可以注入新的生命到盆栽室内植物市场在加拿大。即使只占这些植物市场的10%，也相当于147万株植物。此外，除了与进口和安全法规相关的成本外，从其他国家连续进口种子/幼苗将具有环境污染（微生物和不需要的外来植物物种）的重大风险。为了寻求具有成本效益和可持续的方式在加拿大繁殖具有商业价值的植物，加拿大植物苗圃公司Valleyview Gardens建立了一个植物组织培养设施，用于体外繁殖。最近，他们已经成功地产生了商业上重要的木本植物白含笑（白色檀香）的有活力的愈伤组织。下一步，从这种愈伤组织再生整个幼苗是具有挑战性的，需要植物组织培养专家的帮助。多伦多大学的吉冈实验室在植物组织培养方面有着广泛的专业知识 并配备了应对这一挑战所需的所有基础设施。因此，我们将合作，从M。白色愈伤组织预期建立的方法可以应用于其他商业上重要的木本植物，这将提供一个显着的优势，加拿大植物苗圃业务，以及维护加拿大的环境安全。这一结果在科学上也很重要，因为它可能会促进木本植物组织培养，而目前对许多树种来说，这是很困难的。

Plant nursery techniques have been developed intensely not only for food production, but also for gardening and indoor plants. The plant nursery business is booming with current high demand for indoor plants in homes, offices and public spaces. Currently, Canadians are importing many different plant species from all over the world and are propagating them to meet an ever increasing demand. According to Statistics Canada, in 2013 the Canadian Greenhouse Industry produced a combined 14,700,000 plants of Chrysanthemums and Kalanchoes (the two leading indoor flowering plants). Introduction of a new plant that will compete in the same marketplace as Chrysanthemum and Kalanchoe can breathe new life into the potted indoor plant market in Canada. Even at a mere 10% of the market of these plants, it would equal 1, 470,000 plants. In addition, importing seeds/seedlings continuously from other countries will have a significant risk for environmental contamination (microorganisms and unwanted foreign plant species) in addition to the cost associated with import and safety regulations. To seek cost-effective and sustainable ways to propagate commercially valuable plants in Canada, the Canadian plant nursery company, Valleyview Gardens has established a plant tissue culture facility for in vitro propagation. Recently they have succeeded in generating viable callus tissue of Michelia alba (White Sandalwood) which is a commercially important woody plant. The next step, the regeneration of whole seedling from this callus, is challenging and requires assistance from plant tissue culture specialists. The Yoshioka laboratory at the University of Toronto has extensive expertise in plant tissue culture and is fully-equipped with all infrastructure necessary for this challenge. Thus, we will collaborate for the regeneration of plants from the M. alba callus. The anticipated established method can be applied to other commercially important woody plants and this will provide a significant advantage to the Canadian plant nursery business as well as maintain Canadian environmental safety. The outcome is also scientifically important as it may advance woody plant tissue culture, which is currently difficult for many tree species.