Super Resolution 3D Microscopy for Nano-structured Lab-in-fibre

将开发一个专用的超分辨率倒置显微镜工作站，用于开发（1）光纤实验室，（2）纳米结构表面，（3）智能导管/柔性生物传感器，（4）光学网络电路，（5）薄膜实验室集成电路，以及（6）非线性光学科学和纳米等离子体传感前沿的光学处理工具。 该平台将促进新形式的光纤封装和组装，将我们的激光制造设备与光源，微机电结构和光学传感器互连，同时还促进它们与电气，热以及微和纳米流体控制系统的轻松集成。 强大的工作站将进一步使我们组装的微系统能够通过我们的飞秒激光器进行探测，用于多光子和多光束成像，用于高分辨率应用，包括三维成像。 通过这种方式，我们将能够研究和优化我们在光纤内部形成的复杂纳米结构和光学网络的设计，并遵循微米和纳米工具的先进发展，以研究和操纵活细胞，细菌，蛋白质，最终DNA的尺寸尺度低于100 nm。使用干式、油浸（与玻璃匹配）和水浸透镜进行无失真成像，是在一系列环境和应用中推动光纤实验室研究的必要条件，这些环境和应用与新的学术、医疗和工业支持研究有关。一个模块化倒置显微镜平台将与各种可见光到红外光源互补，填补了我们光谱源和表征工具的空白。 精确的对准电机和先进的成像软件确保了最先进的显微镜将可用，而我们的可重构平台为实时成像诊断提供了广泛的自由度，从单个组装探针平台内对我们的设备或生物物种进行激光纳米加工。 新的研究平台将能够开发用于电信和传感应用的光纤包层光子学，用于基础广泛的实验室，制造，工业和环境监测应用的光流体传感器，以及用于护理点，外科手术和其他医疗程序的新型生物探针和智能导管。

A purpose-build super-resolution inverted microscope station will be developed to serve multiple purposes towards development of (1) Lab-in-fibre, (2) nano-structured surfaces, (3) smart catheters / flexible biosensors, (4) optical network circuits, (5) lab-in-film integrated circuits, and (6) optical processing tools at forefronts of non-linear optical sciences and nano-plasmonic sensing. The platform will facilitate new forms of optical fiber packaging and assembly that interconnect our laser-fabricated devices with optical sources, micro-electromechanical structures, and optical sensors while also facilitating their facile integration with electrical, thermal, and micro- and nano-fluidic control systems. The powerful workstation will further enable our assembled micro-systems be probed by our femtosecond lasers for multi-photon and multi-beam imaging for high resolution applications including three-dimensional imaging. In this way, we will be able to study and optimize the design of the intricate nano-structure and optical networks we have formed inside the optical fibers and follow with advanced development of micro- and nano-tools to study and manipulate live cells, bacteria, protein and ultimately DNA on size scales breaking below 100 nm. Distortion free imaging with dry, oil- (matched to glass), and water-immersion lens are required to drive lab-in-fibre research over a range of environments and applications connected to new academic, medical and industry support research. A modular inverted Microscope Platform will be complemented with various visible to IR sources that fill out gaps in our spectral sources and characterization tools. Precision alignment motors and advanced imaging software ensure state of the art microscopy will be available while our reconfigurable platform provides wide latitude for real-time imaging diagnostics through to laser nano-machining of our devices or biological species from within the single assembly-probe platform. The new research platform will enable development of fiber cladding photonics for telecom and sensing applications, of optofluidic sensors for widely based laboratory, manufacturing, industrial and environmental monitoring applications, and new types of bioprobes and smart catheters for point-of-care, surgical and other medical procedures.