Abberior 3D-STED microscope for super-resolution imaging

用于超分辨率成像的 Abberior 3D-STED 显微镜

• 批准号: 10630881
• 负责人: Avital Adah Rodal
• 金额: $ 123.56万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10630881
• 关键词: 3-Dimensional; Aging; Area; Biological Process; Biology; Cancer Biology; Cells; Cellular Structures; Communities; Confocal Microscopy; Core Facility; Data; Disease; Epilepsy; Faculty; Funding; Health; Human; Image; Image Analysis; Institution; Learning; Malignant Neoplasms; Memory; Microscope; Microscopy; Molecular Structure; Nerve Degeneration; Neurons; Optics; Organelles; Organism; Preparation; Research; Research Personnel; Resolution; Sampling; Science; Signal Transduction; Structure; Synapses; System; Technology; Tissues; United States National Institutes of Health; Universities; base; confocal imaging; experimental study; fighting; imaging system; improved; in vivo; instrument; interest; molecular scale; nanometer; nanoscale; novel therapeutics; prevent; quantitative imaging; subcellular targeting; superresolution imaging; therapeutic nanoparticles

PROJECT SUMMARY We are requesting funds to purchase an Abberior Instruments FACILITY line 3D STED microscope to be maintained in the Brandeis University Confocal Imaging Core Facility. This microscope will serve a primary user group of 10 NIH funded investigators, and two junior faculty who will be applying for NIH funding, and we welcome additional users from the biomedical sciences at Brandeis and the region. Our groups pursue a wide variety of projects relevant to human health including aging, cancer biology, neurodegeneration, epilepsy, learning and memory, and the origins of multicellular organisms. A common feature of these diverse studies is a deep reliance on optical microscopy and an overarching interest in understanding how molecular-scale structures (tens of nanometers in size) control cell and tissue-scale biological processes. A major barrier to our progress in these areas is the lack of an imaging system at our institution that can resolve these tiny structures of interest. Among the many strategies to overcome this resolution barrier (such as STORM, SOFI, and expansion microscopy), a STED microscope is the ideal solution for our research community: It is fundamentally a confocal microscope, and our user base has exceptionally broad and deep expertise in all aspects of confocal microscopy including sample preparation, imaging, and rigorous quantitative image analysis. A STED microscope would represent a five-fold improvement in resolution over our current capabilities and would open up avenues of biology that are completely invisible to us now. Data collected on the instrument will help answer many open questions: How do novel therapeutic nanoparticles target subcellular organelles to fight cancer? How do signal sending and receiving structures at neuronal synapses assemble together to control learning and memory or prevent epilepsy? How do molecules important to aging and neurodegeneration localize and function at these synapses? Because of the small size of each of these structures, these are questions we cannot answer with our current technology. Beyond the increase in resolution this system provides, it is equipped with features that facilitate deep volumetric imaging and live imaging, which will allow us to perform experiments in vivo in living tissues. Together, the Abberior STED system would profoundly improve our ability to discover how nanoscale molecular and cellular structures control cell and tissue biology that is relevant to human health and disease.

项目摘要 我们要求资金购买Abberior Instruments设施线3D STED显微镜为 维护在布兰代斯大学共焦成像核心设施中。该显微镜将为主要用户服务 由10名NIH资助的研究人员组成的小组和两个将申请NIH资金的初级教师，我们 欢迎布兰代斯和该地区生物医学科学的其他用户。我们的团体追求广泛的 与人类健康相关的各种项目，包括衰老，癌症生物学，神经退行性，癫痫， 学习和记忆，以及多细胞生物的起源。这些不同研究的共同特征是 非常依赖光学显微镜和对理解分子尺度的总体兴趣 结构（大小的数十个纳米）控制细胞和组织尺度生物学过程。我们的主要障碍 在这些领域的进步是我们机构缺乏成像系统可以解决这些微小的结构 感兴趣的。在克服这种解决障碍的众多策略中（例如风暴，索非人和 扩展显微镜），sted显微镜是我们研究社区的理想解决方案：从根本上讲是 共聚焦显微镜以及我们的用户群在共焦的各个方面都具有广泛而深厚的专业知识 显微镜，包括样品制备，成像和严格的定量图像分析。一个st 显微镜将比我们当前功能的分辨率提高了五倍，并将开放 现在对我们现在完全看不见的生物学途径。在乐器上收集的数据将有助于回答 许多开放的问题：新型的治疗纳米颗粒如何针对亚细胞细胞器来对抗癌症？如何 在神经元突触中发送和接收结构的信号组装在一起，以控​​制学习和 记忆还是预防癫痫？分子如何对衰老和神经变性重要 在这些突触中？由于每个结构的规模很小，这些问题是我们无法回答的问题 使用我们当前的技术。除了该系统提供的分辨率增加之外，它还配备了功能 这有助于深度容积成像和实时成像，这将使我们能够在体内进行实验 活组织。共同的abberior Sted系统将深刻提高我们发现如何发现的能力 纳米级分子和细胞结构控制与人类健康相关的细胞和组织生物学 疾病。