Abberior 3D-STED microscope for super-resolution imaging

用于超分辨率成像的 Abberior 3D-STED 显微镜

• 批准号: 10630881
• 负责人: Avital Adah Rodal
• 金额: $ 123.56万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10630881
• 关键词: 3-Dimensional; Aging; Area; Biological Process; Biology; Cancer Biology; Cells; Cellular Structures; Communities; Confocal Microscopy; Core Facility; Data; Disease; Epilepsy; Faculty; Funding; Health; Human; Image; Image Analysis; Institution; Learning; Malignant Neoplasms; Memory; Microscope; Microscopy; Molecular Structure; Nerve Degeneration; Neurons; Optics; Organelles; Organism; Preparation; Research; Research Personnel; Resolution; Sampling; Science; Signal Transduction; Structure; Synapses; System; Technology; Tissues; United States National Institutes of Health; Universities; base; confocal imaging; experimental study; fighting; imaging system; improved; in vivo; instrument; interest; molecular scale; nanometer; nanoscale; novel therapeutics; prevent; quantitative imaging; subcellular targeting; superresolution imaging; therapeutic nanoparticles

PROJECT SUMMARY We are requesting funds to purchase an Abberior Instruments FACILITY line 3D STED microscope to be maintained in the Brandeis University Confocal Imaging Core Facility. This microscope will serve a primary user group of 10 NIH funded investigators, and two junior faculty who will be applying for NIH funding, and we welcome additional users from the biomedical sciences at Brandeis and the region. Our groups pursue a wide variety of projects relevant to human health including aging, cancer biology, neurodegeneration, epilepsy, learning and memory, and the origins of multicellular organisms. A common feature of these diverse studies is a deep reliance on optical microscopy and an overarching interest in understanding how molecular-scale structures (tens of nanometers in size) control cell and tissue-scale biological processes. A major barrier to our progress in these areas is the lack of an imaging system at our institution that can resolve these tiny structures of interest. Among the many strategies to overcome this resolution barrier (such as STORM, SOFI, and expansion microscopy), a STED microscope is the ideal solution for our research community: It is fundamentally a confocal microscope, and our user base has exceptionally broad and deep expertise in all aspects of confocal microscopy including sample preparation, imaging, and rigorous quantitative image analysis. A STED microscope would represent a five-fold improvement in resolution over our current capabilities and would open up avenues of biology that are completely invisible to us now. Data collected on the instrument will help answer many open questions: How do novel therapeutic nanoparticles target subcellular organelles to fight cancer? How do signal sending and receiving structures at neuronal synapses assemble together to control learning and memory or prevent epilepsy? How do molecules important to aging and neurodegeneration localize and function at these synapses? Because of the small size of each of these structures, these are questions we cannot answer with our current technology. Beyond the increase in resolution this system provides, it is equipped with features that facilitate deep volumetric imaging and live imaging, which will allow us to perform experiments in vivo in living tissues. Together, the Abberior STED system would profoundly improve our ability to discover how nanoscale molecular and cellular structures control cell and tissue biology that is relevant to human health and disease.

项目概要 我们正在申请资金购买 Abberior Instruments FACILITY 系列 3D STED 显微镜 保存在布兰迪斯大学共焦成像核心设施中。该显微镜将为初级用户服务 由 10 名 NIH 资助的研究人员和两名将申请 NIH 资助的初级教员组成的小组，我们 欢迎来自布兰代斯和该地区生物医学科学领域的更多用户。我们的团队追求广泛 与人类健康相关的各种项目，包括衰老、癌症生物学、神经退行性疾病、癫痫、 学习和记忆，以及多细胞生物的起源。这些多样化研究的一个共同特点是 对光学显微镜的深度依赖以及对了解分子尺度如何进行研究的总体兴趣 结构（尺寸为数十纳米）控制细胞和组织规模的生物过程。我们的一个主要障碍 这些领域的进展是我们机构缺乏可以解析这些微小结构的成像系统 的兴趣。克服这一解决障碍的众多策略（例如 STORM、SOFI 和 膨胀显微镜），STED 显微镜是我们研究界的理想解决方案：它从根本上 共焦显微镜，我们的用户群在共焦的各个方面都拥有极其广泛和深入的专业知识 显微镜包括样品制备、成像和严格的定量图像分析。一个STED 显微镜的分辨率将比我们目前的能力提高五倍，并且将开放 探索我们现在完全看不见的生物学途径。仪器上收集的数据将有助于回答 许多悬而未决的问题：新型治疗性纳米粒子如何靶向亚细胞器来对抗癌症？如何 神经元突触处的信号发送和接收结构是否组装在一起以控制学习和 记忆力还是预防癫痫？对衰老和神经退行性变重要的分子如何定位和发挥作用 在这些突触处？由于每个结构的尺寸都很小，这些是我们无法回答的问题 以我们现有的技术。除了该系统提供的分辨率增加之外，它还配备了以下功能 促进深度体积成像和实时成像，这将使我们能够在体内进行体内实验 活组织。总之，Abberior STED 系统将深刻提高我们发现如何 纳米级分子和细胞结构控制与人类健康和相关的细胞和组织生物学 疾病。