Multimode Plate Reader with Fluorescent Polarization Capabilities

该提案要求资助具有96孔和384孔格式和小体积1536格式荧光偏振（FP）分析能力的平板阅读器。该仪器将作为部门核心设施，用于快速和经济有效地测量生物分子（蛋白质、DNA）和合成小分子之间相互作用中的抑制和解离常数。这些相互作用的测量是化学生物学和有机/生物有机化学研究的中心课题。这些能力对于本提案的所有共同申请人的研究至关重要。下面列出了该仪器在特定研究小组中的具体应用。化学系的直接共同申请人和特定最终用户均列出：

This proposals requests funding for plate reader with capabilities to perform Fluorescent Polarization (FP) assay in 96-well and 384-well format and low-volume 1536-format. The instrument will serve as a Departmental Core Facility for rapid and cost-effective measurement of inhibition and dissociation constants in the interactions between biomolecules (proteins, DNA) and synthetic small molecules. Measurement of these interactions is a central topic of research in Chemical Biology and Organic/Bioorganic Chemistry. These capabilities are critical for the research of all co-applicants on this proposal. Specific applications enabled by this instrument in specific research groups are listed below. Both direct co-applicants and specific end-users in the Department of Chemistry are listed: