Growth and development of the wheat starch granule associated proteome during growth and development of the seed

不断增长的人口依赖谷物作为其主要食物来源。对小麦、水稻和玉米等谷物的成分进行了生化和营养分析，以提高其数量和质量。谷类作物农艺的生长和发展必须得到多个层面的谷物籽粒分析的补充：了解营养决定因素，增强分子机制以提高对病原体和害虫的抵抗力，并使我们能够更好地设计工业过程，如淀粉涂层、麦芽和酿造以及生物燃料。这一谷物蛋白质组学计划以尽可能高的分辨率，蛋白质质谱学，检查了现役作物中七到八周的谷物发育。剖析控制谷物发育，特别是淀粉生物合成的遗传和代谢调控网络的最快方法是确定颗粒表面所有潜在的蛋白质成分。这个项目结合了植物生物学、系统生物学和亚细胞显微镜的原理。这些系列的研究覆盖了一种广泛的蛋白质组学方法，使用高通量质谱学和高效液相色谱相结合的方法。因此，我们提出了具体的研究目的，旨在提高我们对胚乳细胞内颗粒如何生长的理解。该计划也将是植物育种者和食品生物技术专家的重要资源，因为发现的蛋白质可以用于设计非食品和食品行业的改良作物。

The growing human population relies on cereal grains for its major food source. The composition of grains like wheat, rice and maize have been analyzed biochemically and nutritionally to enhance their quantity and quality. Growth and development of the agronomy of cereal crops must be complemented by analysis of the grain kernels at many levels: understand the nutritional determinants, enhance molecular mechanisms to improve resistance to pathogens and pests, and allow us to better engineer industrial processes like starch coatings, malting and brewing and biofuels. This cereal proteomics program examines the seven to eight weeks of grain development in the standing crop at the highest possible level of resolution, protein mass spectrometry. The fastest way to dissect the genetic and metabolic regulatory networks governing grain development and in particular starch biosynthesis is by identifying all potential protein components at the granule surface. This program combines elements of plant biology, systems biology and subcellular microscopy. These series of investigations are overlayed with a broad scope proteomics approach using high-throughput mass spectrometry combined with liquid chromatography. Therefore, we propose specific research aims to improve our understanding of how granules grow inside growing endosperm cells. The program will also be an important resource for plant breeders and food biotechnologists because the discovered proteins can be deployed in the design of improved crops in both the non-food and food industries.