Protocol development to enable high-throughput sequencing of 16S rRNA genes for industry-specific sample analysis

使用可持续的就地生物修复技术来清理被工业化学品污染的场地，需要彻底了解一组有益微生物的活动，这些微生物群体共同努力降解这些污染物。微生物DNA的高通量测序可以提供与污染现场修复相关的多样性和潜在功能的有价值的信息。SiREM(www.sireLab.com)是安大略省圭尔夫市的一家企业，提供测试和产品以支持现场清理。根据拟议的项目，滑铁卢大学研究员Josh Neufeld博士将提供他在分析微生物群落方面的专业知识，以帮助SiREM确定合适的协议，为其客户提供与现场清理相关的自动化微生物分析管道。通过解决SiREM的这一挑战，拟议的研究将通过更好地管理微生物群落来可持续地清理受污染的场地，帮助SiREM创造更清洁的环境。

Using sustainable in situ bioremediation technologies to clean up sites that are contaminated with industrial chemicals requires a thorough understanding of the activities of groups of beneficial microbes that work together to degrade these contaminants. High-throughput sequencing of microbial DNA can provide valuable information about the diversity and potential functions associated with contaminant site remediation. SiREM (www.siremlab.com) is a business in Guelph (Ontario) that provides testing and products in support of site cleanup. Under the proposed project, University of Waterloo researcher Dr. Josh Neufeld will provide his expertise in analyzing microbial communities to help SiREM identify a suitable protocol for providing their clients with an automated microbial analysis pipeline related to in situ site cleanup. By solving this challenge of SiREM, the proposed research will help SiREM produce a cleaner environment through better management of microbial communities to clean up contaminated sites sustainably.