Particle size analysing system for material composites applications in the biomedical engineering field

颗粒 工程学是一个快速发展的领域，需要精确的 特征化工具，以便在该领域工作的研究团队能够 在国际上具有竞争力。几个研究小组在 多伦多大学的研究人员， 颗粒和改变的细胞类型的表征，尺寸范围从纳米到高微米 研究和发现新材料和治疗的规模 用于医疗保健的系统（用于心脏组织、硬组织和 软组织粘合剂，生物合成改变的细胞，牙齿和骨骼 复合材料）需要获得专用的现有技术的颗粒尺寸 分析系统在过去的两年里，首席PI的NSERC发现和工业 协同赠款显着增加了他们的活性在新的聚合物 设计纳米和微米粒子在合作与许多团体在 多伦多大学，这已经在部分兴趣翻译 与两家多伦多大学分拆公司（Interface Biologics）相关的活动 Inc- ORF-RE赠款; Polumiros Inc -NSERC-CIHR协作赠款）。颗粒分析工作需要一个控制污染的系统， 细胞毒性和药物有效载荷性能数据的解释， 以及具有颗粒尺寸通用性的系统 分析（在定义药物剂量、 影响蛋白质和细胞相互作用，并正确识别细胞 来自多细胞类型混合物的表型）。后者对研究至关重要 继续前进Santerre的NSERC Discovery更新在1.5年内将有大约。60%的活性与粒度分析有关， 将有> 20个实习生项目依赖于访问这些 系统.该设备将在Santerre的监督下，位于转化生物学和工程 特德罗杰斯心脏研究中心的TBEP计划（其中5名用户 目前还没有这样的系统）。进入这些系统的重要性 TBEP已承诺为服务合同提供匹配资金，如果获得资助，NSERC将提供15万以上的资金。虽然我们已经获得了有限的访问校园其他系统在过去几年中，他们在高 其他群体的需求，这延误了我们及时接触他们，他们没有 配置为颗粒大小的范围和多样性的化学，我们 各种项目的需求是一致的。确定的系统是 Delsa-Max zeta电位粒度分析（粒度范围为 .3nm-10µm;数据采集以秒为单位，软件分析 复杂和多功能），以及Multi-sizer 4 E（粒度范围 0.4-2000µm;提供颗粒数量和尺寸分布，可用于 用于区分细胞;通用分析）。

Particle engineering is a rapidly emerging field which requires accurate characterization tools in order for research teams working in the area to be competitive at an international level. Several research groups at the University of Toronto who are now involved in engineering biomaterial particulates and the characterization of altered cell types, with sizes ranging from the nano- to the high micron scale for research and discovery of novel materials and therapeutic systems applied in health care (drug delivery for cardiac tissues, hard and soft tissue adhesives, biosynthetically altered cells, dental and bone composites) have a need to access dedicated state-of-the art particle size analyser systems. Over the past two years the lead PI's NSERC Discovery and Industry Synergy grants have significantly increased their activity in novel polymer design for nano and micro-particles in collaboration with many groups at the University of Toronto, and this has been in part of interest to translational activities associated with two U of T spin out companies (Interface Biologics Inc- ORF-RE grant; Polumiros Inc - NSERC-CIHR Collaborative grant). Particle analysis work requires a system controlled for contamination that could affect the interpretation of data on cell toxicity and the performance of drug payloads, as well as systems having versatility for particle size analytics (important in defining drug dosing, electrical charges that influence protein and cell interactions, and properly identifying cell phenotypes from multi-cell type mixtures). The latter are critical for the research to go forward. Santerre's NSERC Discovery renewal in 1.5 years will have approx. 60% of its activity related to particle size analysis, and the combined PIs will have > 20 trainee projects dependent on access to these systems. The equipment will be under Santerre’s oversight and located in the Translational Biology and Engineering Program (TBEP) at the Ted Rogers Centre for Heart Research (where 5 of the users are located and there are currently no such systems). The importance of access to such systems has been recognized by the TBEP who has committed to providing matching funding for service contracts, above the 150K that will be provided by NSERC if funded. While we have gained limited access to other systems on campus over the past few years, they are in high demand by other groups, which delays our timely access to them, and they are not configured to the range of particle sizes and diversity of chemistry that our various projects needs are aligned with. The identified sytems are the Delsa-Max zeta potential particle size analysis (particle size range of .3nm-10µm; data acquisition is in seconds and software analytics are sophisticated and versatile), and the Multi-sizer 4E (particle size range of 0.4-2000µm; provides particle number and size distribution, and can be used for differentiating cells; versatile analytics).