Integrated biosensors

本项目旨在推进一种光学生物传感器平台。这一目标将首先通过台式询问器和生物传感器芯片的概念的设计和建模来实现，然后通过使用市场上可获得的部件、定制的部件以及为询问器控制、数据采集和处理而设计和编写的软件来实现询问器，以及通过在洁净室环境中使用标准半导体制造工具应用晶片规模的微制造工艺来实现生物传感器芯片。询问器和生物传感器芯片的操作将通过应用于与药物发现和护理点诊断相关的生物检测问题来演示，首先是在干净的液体中，然后是在复杂的液体中。将开发用于直接检测、夹心分析、竞争分析和动力学提取的协议，以应用于生物分子相互作用分析(BIA)。将根据患者血液样本(血清、血浆)中可溶生物标记物的检测，制定检测心脏病和甲状腺功能缺乏症等疾病的方案。该项目完成后，由台式询问器、生物传感器芯片、检测方案和生物检测结果组成的成果将转移到加拿大工业界。对加拿大工业的好处包括以演示形式提供新的高性能台式生物检测平台，适用于药物发现和疾病检测应用。验证该平台的生物检测方案和结果将分发给科学界，特别是在生物传感器学科内。通过这个项目形成的HQP将培养在工业界和学术界高度重视的技能。他们将在项目期间与行业合作伙伴互动，并最终参与到向行业转让技术的过程中。

This project aims to advance an optical biosensor platform. This objective will be met, initially through the design and modelling of concepts for a benchtop interrogator and for biosensor chips, then through implementation of the interrogator using commercially-available components, custom-machined parts, and software designed and written for interrogator control, data acquisition and processing, and of the biosensor chips by applying wafer-scale microfabrication processes using standard semiconductor fabrication tools in a cleanroom setting. Operation of the interrogator and biosensor chips will be demonstrated by application to biodetection problems relevant to drug discovery and point-of-care diagnostics, initially in clean fluids, then in complex fluids. Protocols for direct detection, sandwich assays, competitive assays, and kinetics extraction will be developed for application to biomolecular interaction analysis (BIA). Protocols for the detection of diseases, such as heart disease and thyroid deficiency, will be developed, based on the detection of soluble biomarkers in patient blood samples (serum, plasma). Upon completion of the project, the outcomes, consisting of the benchtop interrogator, biosensor chips, detection protocols, and biodetection results will be transferred to Canadian industry. The benefits to Canadian industry include the provision of a new high-performance benchtop biodetection platform in demonstrator form, suitable to drug discovery and disease detection applications. The biodetection protocols and results validating the platform, will be disseminated to the scientific community, particularly within the biosensors discipline. The HQP formed through this project will develop skills that are highly valued within industry and academia. They will interact with the industrial partner over the duration of the project and ultimately participate in technology transfer to industry.