A global analysis of ploidy maintenance in S. cerevisiae

倍性，即生物体中完整染色体的数量，在真核细胞中受到严格调控，对细胞功能和存活至关重要。细胞必须协调多种途径，以确保复制的DNA准确、及时地分离，以防止染色体数量的变化。我们正在采取双管齐下的方法来开发一个机制的理解途径，维持倍性的模式真核生物酿酒酵母。首先，我们发现了SWI/SNF家族染色质重塑因子RSC在倍性维持中的一个意想不到的作用。其次，我们进行了基因组尺度的筛选，以鉴定出芽酵母中倍性维持基因的补体。该筛选发现了蛋白质翻译和倍性维持之间的新关系，我们发现该途径中基因的缺失导致基因组大小增加了四倍。我们现在准备深入研究RSC调控倍性的分子机制，探索细胞形态与倍性的关系，并确定蛋白质翻译与倍性维持之间的关系。

Ploidy, the number of complete sets of chromosomes in an organism, is tightly regulated in eukaryotic cells, and is critical for cell function and survival. Cells must coordinate multiple pathways to ensure that replicated DNA is segregated accurately and in a timely fashion to prevent changes in chromosome number. We are taking a two-pronged approach to develop a mechanistic understanding of the pathways that maintain ploidy in the model eukaryote Saccharomyces cerevisiae. First, we discovered an unanticipated role for the SWI/SNF family chromatin remodeler RSC in ploidy maintenance. Second, we conducted a genome-scale screen to identify the complement of ploidy maintenance genes in budding yeast. This screen identified a novel relationship between protein translation and ploidy maintenance and we discovered that the loss of genes in this pathway caused a quadrupling in genome size. We are now poised to delve into the molecular mechanism by which RSC regulates ploidy, to explore the relationship between cell morphology and ploidy, and to define the relationships between protein translation and ploidy maintenance.