Development of a Standardized Procedure to Assess Total Antioxidant Efficiency of plant-based pharmaceuticals

在生物体中，氧化/抗氧化失衡在不同的病理生理条件下起着关键作用。一般的和特殊的抗氧化剂可以防止生物目标的氧化损伤，抗氧化剂的摄入与发生某些疾病的风险呈负相关。泥盆纪健康集团是一家后期植物制药公司，采用新方法开发药物和包括抗氧化剂在内的制药产品。众所周知，不同的抗氧化剂的还原能力和作用机制各不相同，而不同抗氧化剂的协同作用，例如在植物提取物中，比单独使用化合物提供更大的抗氧化剂保护。已经开发了不同的基于化学的测定方法来测定抗氧化能力。然而，为了研究天然产物潜在的抗氧化健康促进作用，考虑到其体内作用机制的复杂性，单一的体外化学分析不足以评估和比较其抗氧化性能。因此，本项目建议开发一种评估总抗氧化效率的标准化程序（SPATAE），用于筛选泥盆纪样品的抗氧化能力、活性和效果。特别是，我们建议结合化学、生物和细胞检测来评估多种生物水平的抗氧化效率。在选择纳入SPATAE的检测方法时，我们的目标是使用具有明确终点和化学机制的简单方法测量潜在应用中实际发生的化学反应，并且具有良好的运行内和日内重复性。如果没有标准化，植物药及其相关产品的销售就会变得武断，植物药和营养药品的技术发展就会受到阻碍。

In living organisms, the oxidant/antioxidant unbalance plays a pivotal role in different pathophysiologicalconditions. General and specific antioxidants prevent the oxidative damage on biological targets and an intakeof antioxidants is inversely associated with the risk to develop some pathologies.The Devonian Health Group isa late stage botanical pharmaceutical corporation with novel approaches to develop drugs and pharmaceuticalsproducts including antioxidants. It is known that different antioxidants vary in their reducing power andmechanism of action, and the cooperation of different antioxidants, in a botanical extract for example, providesgreater protection against oxidants than compound alone. Different chemical-based assays have been developedto determine antioxidant capacity. However, to study the potential antioxidant health-promoting effects ofnatural products, considering the complexity involved in their in vivo mechanisms of action, a single in vitrochemical assay is not enough to evaluate and compare their antioxidant properties. Therefore, the presentproject proposes to develop a Standardized Procedure to Assess Total Antioxidant Efficiency (SPATAE) thatwill be employed for screening antioxidant capacity, activity and effects of Devonian samples. In particular, wepropose the combination of chemical-, biological-, and cellular-based assay to evaluate antioxidant efficiencyat multiple biological levels. In the selection of the assays to include in the SPATAE, we aim to measurechemistry actually occuring in potential application using simple methods with defined endpoint and chemicalmechanism also good within-run and between-day reproductibility. Without standardization, marketing ofbotanicals ans associated products becomes arbitrary and technological development of botanicalpharmaceuticals and nutraceuticals is hindered.