Model directed phycocyanin over-production in blooming algal cell-factories

调节光合微生物的代谢表达提供了直接从阳光、水和二氧化碳中生产高价值化学物质的可能性。尽管大量的表型andmolecular信息,只有少数蓝藻隔离已经发展成容易处理的遗传systemswith公司相关代谢模型。这限制了可以用作平台化学品生产者的菌株数量。温哥华AlgaBloom国际生物科技公司专门从事bioreactordesign cynaobacterial细胞生长,发展其特定感兴趣的螺旋藻,optimizedfor高增长收益率,作为一个平台化学品生产商来扩展他们的市场范围和盈利能力。通过基因组尺度代谢建模技术和受控实验室生长实验的应用，本项目旨在通过所需的生物合成途径重新定向螺旋藻的代谢物流动。在项目的这一阶段，我们将特别关注藻蓝蛋白的基因组测序和模型定向过度生产，藻蓝蛋白是一种有价值的光合色素，在食品、医疗和仪器仪表行业有多种应用。预测的过度生产表型将在实验和中试规模上进行测试，并将为AlgaBloom开发优化的藻蓝蛋白提取和纯化方案。

Regulating the metabolic expression of photosynthetic microorganisms offers the possibility of producinghigh-value chemicals directly from sunlight, water and carbon dioxide. Despite a plethora of phenotypic andmolecular information, only a few cyanobacterial isolates have been developed into tractable genetic systemswith associated genome-scale metabolic models. This limits the number of strains that can be used as platformchemical producers. AlgaBloom International, a Vancouver-based biotech company specializing in bioreactordesign and cynaobacterial cell growth, is interested in developing their specific strain of Spirulina, optimizedfor high growth yields, as a platform chemical producer to extend their market range and profitability. Throughthe application of genome-scale metabolic modeling techniques and controlled laboratory growth experiments,this project aims to redirect metabolite flow through desired biosynthetic pathways in Spirulina. During thisphase of the project we will specifically focus on genome sequencing and model directed over-production ofphycocyanin, a valuable photosynthetic pigment with multiple applications in the food, medical andinstrumentation industries. Predicted overproduction phenotypes will be tested at both bench- and pilot-scalesand an optimized phycocyanin extraction and purification protocol will be developed for AlgaBloom.