Development of a Procedure for the Extraction and Purification of Rutin from Buckwheat Forage: A Laboratory Study Required for the Industrial Production of Rutin

全天然营养产品（ANNP）有限公司是一家以研究为基础的天然健康产品公司，生产和销售高质量的营养保健品，并获得加拿大卫生部的许可。公司的两位负责人是Ronald R. Marquardt博士和S. Li博士。新成立的ANNP公司有意将两种高价值和广泛使用的天然产品——芦丁及其苷元槲皮素——大量引入营养保健品市场。这些天然产物可以从荞麦饲料中提取。有必要首先证明这两种产品可以在基于实验室的研究中经济地生产，如参与资助申请中所提议的那样。曼尼托巴大学化学系副教授John Sorensen博士将开展一项研究，旨在优化从荞麦中提取、分离和纯化芦丁，然后从芦丁中水解生产槲皮素。Sorensen实验室在天然产物化学方面拥有丰富的经验，并拥有设备齐全的实验室和支持人员，能够开展拟议研究的各个方面。预计该项目将导致开发简单，创新，独特和廉价的方法来生产这两种产品。优化后的程序将在试点工厂研究中扩大规模，随后在马尼托巴省最先进的制造设施中对两种产品进行工业生产。Sorensen博士的研究将使annp能够从当地生产的作物中生产出具有竞争力和盈利的两种高价值营养保健品。这项研究将为在加拿大建立一个新的有利可图的产业奠定基础。

All Natural Nutritional Products (ANNP) Inc. is a research-based Natural Health Product company thatproduces and markets high quality nutraceutical supplements licensed by Health Canada. The two principals ofthe company are Dr. Ronald R. Marquardt and Dr. S. Li. ANNP, in a new venture, is interested in bringing bulkquantities of two high-value and widely used natural products, rutin and its aglycone, quercetin, to thenutraceuticals market. These natural products can be extracted from buckwheat forage. It will be necessary tofirst demonstrate that the two products can be economically produced in a laboratory-based study as proposedin the Engage Grant application. Dr. John Sorensen, Associate Professor in the Department of Chemistry,University of Manitoba, will develop a study designed to optimize the extraction, isolation and purification ofrutin from buckwheat and then the hydrolytic production of quercetin from rutin. The Sorensen lab hasextensive experience in Natural Products Chemistry, and has a well-equipped laboratory and the support staffcapable of carrying out all aspects of the proposed research. It is anticipated that the project will result in thedevelopment of simple, innovative, unique and inexpenseive method for the production of the two products.The optimized procedures will then be scaled up in a pilot plant study followed by the industrial production ofthe two products in a state-of-the-art manufacturing facility in Manitoba. Dr. Sorensen's research will enableANNP to competitively and profitably produce the two high value nutraceuticals from a locally produced crop.The research will form the basis for establishing a new and profitable industry in Canada.