Development of a mass spectrometry-based foodomics approach to characterize the fate of selected antibiotic residues during food processing and storage.

尽管全球都在努力管理作为兽药使用的抗生素，但在肉类、海鲜、牛奶、蜂蜜或鸡蛋等食品中经常发现抗生素残留。最近，我们发现一种抗生素，氯霉素，不仅作为母体化合物存在于食物中，而且还作为多种（通常是未知的）代谢物存在，并且可以在烹饪过程中转化为更复杂的化学混合物，对人类健康的影响未知。***这项研究计划的长期目标是证明许多化学污染物不是单独作为母体化合物存在于食品中，而是作为一种复杂的混合物，通常是由于热降解/异构化/与食品成分相互作用的结果。该计划旨在开发一种新的食品组学方法，以提高从田间到餐桌的食品中化学污染物的检测和识别。这项工作将丰富现有已知污染物（如兽药）及其代谢物和其他结构相关化学品（如降解产物）的数据库。虽然目前的方法适用于任何类型的食物，但最初的努力将集中在鱼/海鲜上，以演示概念方法。***该计划的短期目标（5年）是描述发生的海鲜样品（罗非鱼/鳟鱼/龙虾）中关键兽药残留的命运。申请人打算开发这些鱼/海鲜中三种兽药（硝基呋喃酮、土环素和磺胺二甲氧嘧啶）代谢物的非靶向分析方法（AIM 1）；并研究食物储存和加热（烹饪）对这些残留物造成的变化（AIM 2）。该研究项目将建立海鲜抗生素的最佳食物组学方案，从而为研究其他食物基质和污染物铺平道路（长期目标）。***该项目将在食品化学、分析化学和食品安全领域建立创新和深化知识。值得注意的是，本研究将允许制定海鲜组织代谢组学的分析标准，因为迫切需要标准协议。AIMs 1和AIMs 2的结果还将突出食品中代谢物和降解副产物的多样性，这一点目前仍未确定。综上所述，本研究将对兽药的监测和食品安全风险评估产生影响。预期它将加强和补充管理机构在确定和评估化学品风险方面的工作。

Despite the global efforts to manage antibiotics used as veterinary drugs, residues are frequently detected in food such as meat, seafood, milk, honey or eggs. Recently, we showed that one antibiotic, chloramphenicol, does not exist solely as the parent compound in food, but also occur as a multitude of (often unidentified) metabolites, and can transform during cooking into far more complex chemical mixtures with unknown impact for human health.***The long-term objective of this research program is to prove that many chemical contaminants are not solely present as the parent compound in food, but rather as a complex mixture of often unidentified structures as a result of thermal degradation/isomerization/interaction with food components. This program intends to develop a novel foodomics approach that improves the detection and identification of chemical contaminants in food from field to fork. This work will enrich the current database of known contaminants (e.g. veterinary drugs) with their metabolites and other structurally related chemicals (e.g. degradation products). While the present approach is applicable to any type of food, the initial efforts will focus on fish/seafood to demonstrate the conceptual approach.***The Short-term Objective of this program (5-years) is to characterize the fate of key veterinary drug residues in incurred seafood samples (tilapia/trout/lobster). The applicant intends to develop analytical methods for the non-targeted analysis of the metabolites of three veterinary drugs (nitrofurazone, oxytetracycline and sulfadimethoxine) in these fish/seafood (AIM 1); and investigate the changes induced by food storage and heating (cooking) on these residues (AIM 2). This research program will establish optimal foodomics protocols for antibiotics in seafood, thus paving the way for investigating other food matrices and contaminants (long-term objective).***This project will build innovation and deepen knowledge in the field of food chemistry, analytical chemistry and food safety. Notably, this study will allow developing analytical standards for seafood tissue metabolomics as standard protocols are urgently needed. The results of AIMs 1 and 2 will also highlight the diversity of metabolites and degradation by-products in food, which currently remains unidentified. Overall, this research will have an impact on the surveillance and the food safety risk assessment of veterinary drugs. It is anticipated to strengthen and complement the work of regulatory bodies in the identification and assessment of chemical risks.