Integrated biosensors

该项目旨在推进光学生物传感器平台。这一目标将首先通过设计和建模台式询问器和生物传感器芯片的概念来实现，然后通过使用商用组件、定制加工部件和为询问器控制、数据采集和处理设计和编写的软件来实现询问器，并通过在洁净室环境中使用标准半导体制造工具应用晶圆级微制造工艺来实现生物传感器芯片。询问器和生物传感器芯片的操作将通过应用于与药物发现和即时诊断相关的生物检测问题来演示，最初是在清洁液体中，然后是在复杂液体中。将开发用于生物分子相互作用分析（BIA）的直接检测、夹心测定、竞争测定和动力学提取方案。将根据对患者血液样本（血清、血浆）中可溶性生物标志物的检测，制定心脏病和甲状腺缺乏症等疾病的检测方案。项目完成后，包括台式询问器、生物传感器芯片、检测协议和生物检测结果在内的成果将转移到加拿大工业界。对加拿大工业的好处包括提供一种新的高性能台式生物检测平台，以演示形式，适用于药物发现和疾病检测应用。验证该平台的生物检测方案和结果将传播给科学界，特别是在生物传感器学科内。通过该项目形成的HQP将培养工业界和学术界高度重视的技能。他们将在项目期间与工业合作伙伴互动，并最终参与技术向工业的转移。

This project aims to advance an optical biosensor platform. This objective will be met, initially through the design and modelling of concepts for a benchtop interrogator and for biosensor chips, then through implementation of the interrogator using commercially-available components, custom-machined parts, and software designed and written for interrogator control, data acquisition and processing, and of the biosensor chips by applying wafer-scale microfabrication processes using standard semiconductor fabrication tools in a cleanroom setting. Operation of the interrogator and biosensor chips will be demonstrated by application to biodetection problems relevant to drug discovery and point-of-care diagnostics, initially in clean fluids, then in complex fluids. Protocols for direct detection, sandwich assays, competitive assays, and kinetics extraction will be developed for application to biomolecular interaction analysis (BIA). Protocols for the detection of diseases, such as heart disease and thyroid deficiency, will be developed, based on the detection of soluble biomarkers in patient blood samples (serum, plasma). Upon completion of the project, the outcomes, consisting of the benchtop interrogator, biosensor chips, detection protocols, and biodetection results will be transferred to Canadian industry. The benefits to Canadian industry include the provision of a new high-performance benchtop biodetection platform in demonstrator form, suitable to drug discovery and disease detection applications. The biodetection protocols and results validating the platform, will be disseminated to the scientific community, particularly within the biosensors discipline. The HQP formed through this project will develop skills that are highly valued within industry and academia. They will interact with the industrial partner over the duration of the project and ultimately participate in technology transfer to industry.