Development of an innovative bioelectrochemical process for the degradation of petroleum hydrocarbon contaminated soils/water : b) Enzymatic mode

这个研究项目是基于一个学院-大学-产业合作。它旨在满足TechnoRem公司的需求，这是一家专门从事地下水和土壤处理的公司。目前，受石油烃污染的土壤和地下水是科学家面临的主要挑战。该研发项目的目标是利用CTE （Gherrou, Daghrir & Huot）研究人员的专业知识和INRS-ETE教授SK. Brar的专业知识，开发一种利用生物电化学过程的创新方法，该过程涉及酶促反应和电化学氧化技术的协同作用，用于降解污染土壤和地下水的复杂碳氢化合物链（多环芳烃，C10-C50等）。我们的跨机构团队（INRS-CTE）提供的研发活动可分为三个主要活动。第一部分包括确定合适的阳极和阴极，从而优化其碳氢化合物的氧化能力。将在CTE的实验室规模上对碳氢化合物的电化学氧化能力进行优化。第二项任务将是确定和开发高效酶的纳米配方，并具有在INRS氧化降解碳氢链的能力。在不同的实验条件（pH，温度等）下，将研究酶促过程的效率。本工作的第三部分将集中于开发和应用一种混合生物电化学工艺降解污染土壤和地下水的碳氢化合物（CTE-INRS）。在应用每个过程（酶和电化学）期间确定的最佳处理条件将先验地应用。该项目将受益于电化学氧化技术和酶促工艺相结合的独特专业知识，以及最前沿的研发运营基础设施；及时实现项目目标所必需的两个参数

This research project is based on a college-university-industry collaboration. It aims to meet the needs of TechnoRem Inc., a company specializing in the treatment of groundwater and soils. Nowadays, soil and groundwater contaminated with petroleum hydrocarbons is a major challenge facing scientists. The objective of this R & D project is to leverage the expertise of researchers from CTE (Gherrou, Daghrir & Huot) and the expertise of Professor SK. Brar from INRS-ETE to develop an innovative method using bioelectrochemical process involving synergy of enzymatic reactions and electrochemical oxidation techniques for the degradation of complex hydrocarbon chains (PAHs, C10-C50, etc) that contaminate soil and groundwater. R & D activities offered by our inter-institutional team (INRS-CTE) can be divided into three main activities. The first part comprises identification of the anode and cathode that is appropriate and hence optimize their oxidation capacity of hydrocarbons. An optimization of electrochemical oxidation capacity of hydrocarbons will be performed at laboratory scale at CTE. The second task will be to identify and develop nano-formulations of efficient enzymes and having a capacity for oxidative degradation of hydrocarbon chains at INRS. The efficiency of the enzymatic process will be studied under different experimental conditions (pH, temperature, etc.). The third part of this work will be focused on the development and application of a hybrid bioelectrochemical process for the degradation of hydrocarbons that contaminate soil and groundwater (CTE-INRS). The optimum processing conditions determined during the application of each process (enzymatic and electrochemical) will be applied a priori. This project will benefit from a unique expertise combining electrochemical oxidation techniques and enzymatic processes, and an R & D operational infrastructure at the forefront; two parameters essential for the achievement of the project objectives in time.**