Applying split TreA technology to detect antigen-specific antibodies in colostrum

母源抗体对于保护小牛免受大量新生儿疾病的侵害至关重要。在牛中，** 抗体的母体转移仅限于初乳的摄取，而其他动物则 ** 能够胎盘转移。因此，初乳中的抗体浓度对小牛的存活和健康至关重要。乳制品生产商可以选择在农场测试和储存高质量的 ** 初乳，或购买商业上可获得的（例如萨斯卡通初乳有限公司（SCCL））** 质量受控的冻干初乳。在之前的Engage项目中，我们验证了一种新技术，用于 ** 检测初乳和血清样本中的总IgG，供SCCL的质量控制实验室使用。为了保护小牛免受威胁其健康的病原体的侵害，例如与腹泻和呼吸道疾病相关的病原体，病原体特异性抗体的水平与总量同样重要。目前，为了 ** 确定初乳中这些病原体特异性抗体的范围和保护水平，SCCL需要 ** 与外部实验室合作运行ELISA。在这个项目中，我们将采用以前用于总IgG检测的技术，也定量初乳中的 ** 病原体特异性抗体。该技术基于与病原体特异性蛋白抗原融合的分裂酶。它的优点是不需要冲洗步骤，并且可以定制以 ** 包括不同病原体的抗原。本项目的具体目标是：1.在我们的生物制剂中掺入病原体特异性抗原。2.**对采用ELISA预检测的初乳盲板进行改进和检测验证。**该项目将评估用一种新的 ** 技术检测初乳中病原体特异性抗体的可行性，并将产生可供SCCL使用的检测方法。这些测试将允许SCCL使用 ** 特异性抗体测试结果来为其产品贴上优质标签，并使该公司比竞争对手具有营销 ** 优势。

Maternal antibodies are crucial in the protection of calves against a large number of neonatal diseases. In cattle,**maternal transfer of antibodies is restricted to the uptake of colostrum in contrast to other animals that are**capable of placental transfer. Consequently, the concentrations of antibodies in colostrum is essential for the**survival and well-being of the calves. Dairy producers have a choice of testing and storing high quality**colostrum on farm or purchasing commercially available (e.g. Saskatoon Colostrum Company Ltd. (SCCL))**quality controlled freeze dried colostrum. In a previous Engage project, we validated a novel technology for**testing total IgG in colostrum and serum samples for use in SCCL's quality control lab.**To protect calves from pathogens that threaten their health, such as those associated with scours and respiratory**disease, the levels of pathogen-specific antibodies are equally important as the total amount. Currently, to**determine the range and protective levels of these pathogen-specific antibodies in colostrum, SCCL needs to**collaborate with an external lab to run ELISAs.**In this project, we will adapt the technology previously used for total IgG detection to also quantify**pathogen-specific antibodies in colostrum. This technology is based on a split enzyme fused to pathogen**specific protein antigens. It has the advantage that no rinsing steps are required and that it can be customized to**include antigens of different pathogens.**The specific objectives of this project are: 1. Incorporation of pathogen-specific antigens in our bioreagents. 2.**Improvement and assay validation on blinded panels of colostrum pretested with ELISAs.**This project will assess the feasibility of detecting pathogen-specific antibodies in colostrum with a novel**technology and it will result in tests readily available for use by SCCL. These tests will allow SCCL to use**specific antibody testing results for premium labeling of their product and give the company marketing**advantages over competitors.