CAPILLARY ELECTROPHORESIS INSTRUMENT FOR DEVELOPMENT OF DISRUPTIVE BIOANALYTICAL TECHNOLOGIES

所需设备。Krylov教授的研究旨在开发基于毛细管电泳法(CE)的颠覆性生物分析和生物医学技术。毛细管电泳法是一种通过在毛细管两端施加非常高的电压，在狭窄的毛细管中以无与伦比的高效分离分子的技术。如果没有自动化的毛细管电泳仪，基于CE的生物分析和生物医学技术的发展是不可能的，该仪器可以：(I)分离处于非变性状态的生物分子，如蛋白质、DNA和RNA，(Ii)即使在非常低的浓度下也能可靠地检测它们，以及(Iii)在毛细管端将它们收集成单独的组分。本赠款申请请求为获得这类文书提供支助。拟议的设备将成为克里洛夫教授实验室的主要研究工具，其100%的能力将由克里洛夫教授的团队及其合作者利用。*研究活动将通过所需的设备来实现。该仪器将用于在三个领域取得重大技术进步。第一个领域是识别和验证经典小分子药物的先导。第二个领域是生物药物的发现和质量控制，生物药物是从细胞中获得的大分子药物。第三个领域是非基因组癌症生物标记物的发现、验证和临床应用，例如最近发现的细胞过程调节因子microRNAs。这三个领域的研究是由公认的可用于开发小分子药物、生物制药和非基因组癌症生物标记物的技术缺陷所推动的。该研究计划建立在多个外部支持的研究项目之上，这些项目是几个学术团体、制药公司和医院的合作努力。*研究活动为什么以及对谁来说是重要的。预期的结果将通过简化药物开发的早期阶段而使加拿大制药公司受益。它们还将为加拿大癌症医院提供新的、高度可用的分析工具，用于癌症检测和癌症治疗反应的预测，从而使它们受益。最终，这一结果将使加拿大经济和加拿大人受益，他们将获得新药和新的癌症检测方法。由拟议工具支持的研究涉及各级受训人员：本科生、研究生和博士后研究员。它将为他们提供一个独特的机会，通过先进的分析仪器获得宝贵的实践经验。要获得的技能与现代制药业和癌症护理提供者高度相关。*延迟采购所需设备的影响。无法获得拟议的设备将阻碍克里洛夫教授高效的研究计划，并阻止他的研究生和博士后研究员完成他们的培训。

Requested equipment. Prof. Krylov's research aims at the development of disruptive bioanalytical and biomedical technologies which are based on capillary electrophoresis (CE). CE is a technique that separates molecules with unbeatably high efficiency in a narrow capillary by applying a very high voltage to its ends. The development of CE-based bioanalytical and biomedical technologies is impossible without an automated CE instrument which can: (i) separate biomolecules, such as proteins, DNA, and RNA in their non-denatured states, (ii) reliably detect them even if they are at very low concentrations, and (iii) collect them into separate fractions at the capillary end. The present grant application requests support for acquiring such an instrument. The proposed equipment will be the main research tool in Prof. Krylov's laboratory, and 100% of its capacity will be utilized by Prof. Krylov's group and its collaborators.***Research activities to be enabled by requested equipment. The instrument will be used to make major technological advances in three areas. The first area is identification and validation of leads for classical small-molecule drugs. The second area is discovery and quality control of biopharmaceuticals, which are large-molecule drugs obtained from cells. The third area is discovery, validation, and clinical use of non-genomic cancer biomarkers, such as microRNAs, recently discovered regulators of cellular processes. The research in these three areas is motivated by well-recognized deficiencies of technologies available for development of small-molecule drugs, biopharmaceuticals, and non-genomic cancer biomarkers. The research program is built upon multiple externally supported research projects that are collaborative efforts of several academic groups, pharmaceutical companies, and hospitals.***Why and to whom the research activities are important. The expected outcomes will benefit Canadian pharmaceutical companies by streamlining early stages of drug development. They will also benefit Canadian cancer hospitals by providing them with novel highly-enabling analytical tools for cancer detection and prediction of cancer response to treatments. Eventually, the outcomes will benefit the Canadian economy and Canadians who will be receiving new drugs and new modalities of cancer detection. The research supported by the proposed instrument involves trainees at all levels: undergraduate students, graduate students and postdoctoral fellows. It will provide them with a unique opportunity to gain valuable hands-on experience with advanced analytical instrumentation. The skills to be obtained are highly relevant to the modern pharmaceutical industry and cancer-care providers.***Impact of a delay in acquisition of the requested equipment. The inability to obtain the proposed equipment will stall Prof. Krylov's highly productive research program and stop progress of his graduate students and postdoctoral fellows towards completion of their training.**