New anti-biofilm formulations and products

医疗器械上生物膜的形成可能会导致持续性感染。生物膜的主要特征是它们**自己产生的胞外聚合物(EPS)，起到保护屏障的作用，防止杀菌剂**渗透。凯恩生物技术公司一直致力于生物膜的去除和扩散。凯恩生物技术公司有一种**专有酶，可以用来分解和分散生物膜。他们对开发**在去除工业和医疗保健领域的生物膜方面具有更高效力的新产品感兴趣。我的研究团队**已经成功地使用通过简单工艺合成的氧化铁磁性纳米颗粒(MNPs)**作为“屏蔽剂”，对生物膜EPS的完整性造成负面影响，并导致生物膜脱落。用耐甲氧西林金黄色葡萄球菌(MRSA)菌株验证MNPs的疗效。在旋转磁场下，30 mg/mL11 nm MNPs处理15min后，MRSA生物膜的对数降解率最高(4.71log)。MNPs会对基质造成强烈的机械破坏，并导致生物膜扩散。交变磁场和旋转磁场均能改善MNPs对生物膜的损伤作用。由于AC**场中的热效应导致MNPs溶液温度升高，导致生物膜扩散，而MNPs在旋转磁场中对生物膜造成物理破坏。**我们假设，我们实验室合成的MNPs与凯恩生物技术公司专有的**抗生物膜酶相结合将产生具有协同抗生物膜活性的新配方和产品。这可以转化为凯恩可以商业化的新的抗生物膜产品。

Biofilm formation on medical devices can cause persistent infections. The main feature of biofilms is their**self-produced extracellular polymeric substances (EPSs) that act as a protective shield to prevent biocide**penetration. Kane Biotech Inc. has been working on biofilm removal and dispersal. Kane Biotech has a**proprietary enzyme that can be used to break down and disperse biofilms. They are interested in developing**new products with higher potency in removing biofilms in both industrial and healthcare sectors. My research**group has successfully used Iron oxide magnetic nanoparticles (MNPs), synthesized through a simple process,**as "shield breakers" to negatively impact the integrity of biofilm EPS and cause biofilm detachment. The**Methicillin-resistant Staphylococcus aureus (MRSA) strain was used to demonstrate the efficacy of MNPs. The**highest logarithmic reduction (4.71 log) of MRSA biofilm was achieved after the treatment with 30 mg/mL of**11 nm MNPs under rotating magnetic field for 15 minutes. MNPs caused strong mechanical disruption to the**matrix and led to biofilm dispersal. Both alternating and rotating magnetic fields improved the effect of MNPs**on biofilm damage. The increased MNPs solution temperature caused by the hyperthermic effect in the AC**field led to biofilm dispersal while the MNPs caused physical damage on biofilms in a rotating magnetic field.**We hypothesize that combination of the MNPs synthesized in our lab with Kane Biotech's proprietary**anti-biofilm enzyme will lead to new formulations and products with synergistic anti-biofilm activity. That can**be translated to new anti-biofilm products that Kane can commercialize.