Characterization of Airborne Cat Dander in a Cat Allergen (Fel d1) Exposure Chamber

Red Maple Trials（渥太华，ON）创建了一个新的设施，其中有几个测试室，患者可以接触过敏原（花粉和Fel d1猫过敏原）。在该设施中，可以以受控的方式监测患者的症状（有或没有 ** 药物干预）。该公司有兴趣获得关于过敏原（附着在颗粒物上的Fel d1，通常是猫 ** 皮屑）的分散以及及时监测颗粒物和浓度的更多 ** 详细信息，以更好地控制临床研究期间患者暴露于过敏原的一致性。空气中猫皮屑浓度将使用TSI粒度分布分析仪原位测定，而与 ** 猫皮屑颗粒相关的空气中Fel d1水平将通过安德森级联撞击器收集。同时，将在玻璃纤维过滤器上收集微粒样品（使用Gillian 5000采样泵以稳定流速提取 **）。目前的 ** 项目还将提供对Fel d1进行具有成本效益的ELISA测定的可行性研究。最后，将使用 ** 雷诺平均纳维-斯托克斯（RANS）方程（用于空气（主流））和拉格朗日随机游走 ** 模型（用于颗粒相）对猫过敏原暴露室中的气溶胶扩散进行 ** 数值模拟。

Red Maple Trials (Ottawa, ON) created a new facility with several testing rooms, where patients can be**exposed to allergens (pollens and Fel d1 cat allergen). In this facility, the patient's symptoms (with or without**medication intervention) can be monitored in a controlled manner. The company is interested in obtaining more**detailed information regarding the dispersion of allergens (Fel d1 attached to particulate matter, normally cat**dander), and the monitoring of particulate matter and concentration in time, to better control the consistency of**exposure to allergens by the patients during clinical studies. Airborne cat dander concentration, will be**determined in-situ using a TSI Particle Size Distribution analyzer, while airborne Fel d1 levels associated to the**cat dander particles will be collected via an Anderson Cascade Impactor. In parallel, particle samples (extracted**using a Gillian 5000 sampling pump at a steady flow rate) will be collected on a glass fiber filters. The current**project will also provide the feasibility study of performing a cost-effective ELISA assaying of Fel d1. Finally,**numerical simulation of aerosol dispersion in the cat allergen exposure chamber will be performed using**Reynolds Averaged Navier-Stokes (RANS) equations for the air (primary flow) and Lagrangian random-walk**models for the particulate phase.