Genome sequencing and comparative analysis of brewing yeast cultures

在加拿大和国际上，自酿和精酿啤酒在啤酒行业中所占的比例越来越大。由于小规模、定制和工艺酿造的增加，对混合酵母培养物和混合培养物中与特定风格和风味相关的菌株特征的需求增加。该项目将通过基因组DNA测序来分析不同的酵母混合培养物，以确定混合培养物中与特定风味和特性相关的基因和基因型，以及允许稳定生长和可预测成分的特性。此外，该项目将开发一种基于测序的方法来跟踪混合酵母培养物的长期稳定性，作为质量控制步骤，以确保混合酵母培养物随着时间的推移是一致的。测序将使用Illumina和牛津纳米孔技术（ONT）测序。该项目将评估ONT测序作为微生物测序的可能内部资源，可能提供一个经济实惠的长读测序平台，具有快速的周转时间和比其他测序技术低得多的资本成本。

Homebrewing and craft brewing represent an increasingly large portion of the beer industry in Canada and internationally. As a result of the increase in small scale, custom, and craft brewing there is an increased demand for both mixed yeast cultures and characterization of strains within mixed cultures associated with particular styles and flavours. This project will profile different mixed cultures of yeast through genomic DNA sequencing to identify genes and genotypes associated with particular flavours and properties within mixed cultures, along with properties that allow for stable growth and predictable composition. Additionally, the project will develop a sequencing-based methodology to track the long-term stability of mixed yeast cultures as a quality control step to ensure that mixed culture yeast products are consistent over time. Sequencing will be done using Illumina and Oxford Nanopore Technology (ONT) sequencing. This project will assess ONT sequencing as a possible in-house resource for microorganism sequencing, potentially providing an affordable long-read sequencing platform with a rapid turnaround time and a much lower capital cost than other sequencing technologies.