Towards Functional Peptide-Based Supramolecular Structures

以功能肽为基础的超分子结构*******受自然复杂蛋白质系统的启发，我们的研究计划旨在开发以多肽为分子框架的功能超分子结构。对于下一个资助期，我们建议将重点放在四个方面：***1。利用人工离子通道作为传感器件****我们将继续开发基于冠醚肽的离子通道用于传感器件。将在n端位置引入几个生物识别元件来研究并最终检测特定生物标志物和细胞的存在。* * * 2。制备氟化通道和纳米孔****我们将合成氟化螺旋肽，并研究它们在氟-氟相互作用驱动下的自组装成新的超分子结构。这样的上层结构将产生衬有氟的通道和纳米孔。我们打算研究这些氟化通道的膜离子传输特性，以了解离子转运所必需的结构特征。开发抗菌肽纳米结构****针对耐药细菌的新型高效抗菌药物，我们采用了基于简单的14残基肽的从头方法。我们建议系统地设计具有特定官能团的模型肽，并研究其对重要病原体的抗菌活性。将评估末端位置和序列中的修饰对生物活性的影响。水中仿生超分子催化****为了有效催化工业上相关的水中有机转化，我们提出合成多种可以作为超分子催化剂的肽结构，从而模仿天然酶。我们将研究几种肽类系统；从小的刚性环状肽，到构象偏倚的寡肽。不同的转化将作为模型反应来研究。影响效率的因素将被确定，目标是描述设计原则，以开发用于水中各种各样转化的仿生催化剂。

Towards Functional Peptide-Based Supramolecular Structures*******Inspired by natural sophisticated proteic systems, our research program aims at developing functional supramolecular structures exploiting polypeptide as molecular framework. For the next grant period, we propose to devote our efforts into four areas:***1. Use artificial ion channels as sensing devices****We will pursue our development of crown ether peptide-based ion channels towards sensing devices. Several biological recognition elements will be introduced at the N-terminal position to study and eventually to detect the presence of specific biomarkers and cells. ***2. Preparing fluorinated channels and nanopores ****We will synthesize fluorinated helical peptides and study their self-assembly into novel supramolecular architectures driven by fluorine-fluorine interactions. Such superstructures will create fluor-lined channels and nanopores. We intend to study membrane ion transport properties of those fluorinated channels to understand the structural features essential for ion translocation.***3. Developing antimicrobial peptide nanostructures****Towards novel and efficient antimicrobials against resistant bacteria, we have used a de novo approach based on a simple 14 residue peptide. We propose to engineer systematically this model peptide with specific functional groups and to study their antimicrobial activities towards important pathogens. Modifications at terminal positions and in the sequence will be evaluated with respect to their impact on bioactivity.***4. Biomimetic supramolecular catalysis in water****To catalyze efficiently industrially relevant organic transformations in water, we propose to synthesize a variety of peptide structures that can acts as supramolecular catalysts, hence mimicking natural enzymes. We will investigate several peptidic systems; from small rigid cyclic peptides, to conformationally biased oligopeptides. Different transformations will be studied as model reactions. Factors influencing efficiency will be identified with the goal of delineating design principles for developing biomimetic catalysts for a huge variety of transformations in water.