Development of functionalized magnetic nanoparticles to isolate and detect bacteria

检测细菌，如E.大肠杆菌、军团菌和沙门氏菌是一个全球性问题，对水体和农场的管理很重要。政府和其他政府机构、私营企业和个人寻求水质信息，以制定公共卫生和政策以及商业决策。例如，这些信息可能阻止了有史以来最严重的E。安大略省布鲁斯县的大肠杆菌污染2010年，2,300人患病，7人死亡（来源：加拿大CBC新闻）。目前没有可用的解决方案来检测现场供水中的微生物污染物。BioInk实验室正在与学术团队合作解决这个问题，并开发一种真实的实时检测细菌的现场测试点。具体来说，该项目将开发一种技术，使用涂有捕获剂的磁性纳米颗粒来靶向水样中存在的细菌，随后分离病原体以检测这些细菌中存在的ATP。通过这种检测方法，样品的颜色变化将确认目标细菌存在于其中。该项目完成后，通过将ATP传感器整合到可行的传感器中，该项目将提供一种真实的技术，并允许该公司为全球市场创建一种针对液体传播细菌的解决方案。市政、废水处理设施、政府机构、企业和个人用户将能够使用该产品直接评估其社区或私人威尔斯的水质，以真实的时间。

Detection of bacteria, such as E. coli, Legionella and Salmonella is a global problem important for the management of water bodies and agricultural farms. Municipalities and other government agencies, and private enterprises and individuals seek water quality information to make public health and policy, and business decision. For instance, such information might have prevented the worst-ever outbreak of E. coli contamination in Bruce County, Ont. in 2010 when 2,300 people fell ill and seven died (Source: CBC News, Canada). There is no current solution available that detects microbial contaminants in the water supply at the point of site. BioInk labs is partnering with the academic team to address this problem and develop a point of site test that detects bacteria in real time. Specifically, the project will develop a technology that uses magnetic nanoparticles coated with capture agents to target bacteria present in a water sample and subsequently isolate the pathogens to detect the ATP present in these bacteria. With this detection method, a color change of the sample will affirm that the target bacteria are present in it. Upon completion, the project will provide a real-world technology by incorporating the ATP sensor into a viable sensor and allow the company to create a solution for liquid borne bacteria for the global market. Municipalities, wastewater treatment facilities, government agencies, businesses, and individual users will be able to use the product to directly assess water quality in their communities or private wells in real time.