I2I Phase I: Reagentless sensor technologies for peripheral biochemical monitoring

可穿戴传感器系统为持续监测个人的生命体征和健康状况提供了独特的机会。这些系统可以集成到常见物品中，如衣服，隐形眼镜，绷带和手表，以检测和定量粘膜或生物液体的分析物。完全实现这些系统的一个主要挑战是开发平台，允许对复杂生物分子（如蛋白质、核酸和代谢物）进行实时无试剂分析。电化学传感器是基于廉价仪器和直接测量电流的环境或生物监测的强大平台。由于可以可靠地测量小（nA）电流，因此低检测阈值是可行的。它在实际设备中的实施和对所有类型的蛋白质分析物的推广仍然存在一些挑战。我们最近开发了一种无酶和无试剂的电化学报告系统，该系统显示出适合分析生物样品的灵敏度水平，而无需预处理。在这个项目中，我们将把这些电化学传感器集成到生物相容性探针中，用于原位生化监测。我们将在糖尿病动物模型中测试开发的设备，展示持续和无试剂监测间质液中的胰岛素水平。该项目将开发和测试控制可穿戴监控设备所需的硬件和软件。该项目的可交付成果将对加拿大人口产生重大影响，对监测慢性疾病医疗保健中的持续生物标志物监测健康产生潜在影响。

Wearable sensor systems present a unique opportunity for the continual monitoring of vital signs and health status of an individual. These systems can be integrated into common items such as clothing, contact lenses, bandages, and watches to detect and quantify analytes from mucosal membranes or biological fluids. One major challenge to fully realize these systems is the development of platforms that allow for reagent-free analysis of complex biomolecules such as proteins, nucleic acids, and metabolites in real time. Electrochemical sensors are a powerful platform for environmental or biological monitoring based on inexpensive instrumentation and straightforward measurements of electrical currents. As small (nA) currents can be reliably measured, low detection thresholds are feasible. Several challenges remain for its implementation in practical devices and generalized for all types of protein analytes. We recently developed an enzyme-free and regent-free electrochemical reporter system that exhibits levels of sensitivity suitable for analysis of biological samples without the need for pre-processing. In this project we will integrate these electrochemical sensors into biocompatible probes for in situ biochemical monitoring. We will test the developed device in an animal model of diabetes demonstrating continual and reagent free monitoring of insulin levels from interstitial fluids. Requisite hardware and software for controlling the wearable monitoring device will be developed and tested in the project. The project deliverables will have a large impact on the Canadian population with potential impact in monitoring the health of providing continual biomarker monitoring in healthcare for chronic conditions.