Critical Replacement of a Low-Resolution Mass Spectrometer

这项申请是为购买一台低分辨率质谱仪提供资金。质谱仪是用来测定分子相对分子质量的重要研究仪器。滑铁卢大学质谱学设施(UWMSF)中的一台质谱仪是一台名为Thermo LTQ的低分辨率仪器。它被研究生和博士后研究员用来在不需要高分辨率质谱学数据时进行快速常规测量。HQP还使用它来开发合适的分析条件，然后在该设施的更复杂(和脆弱且昂贵)的高分辨率质谱仪上获取高分辨率MS数据。这种条件的预筛选非常重要，因为它延长了更昂贵的高分辨率仪器的寿命，并降低了维修和维护成本。Thermo LTQ仪器于2013年从多伦多玛格丽特公主医院获得使用，已有16年的历史。它只能在电喷雾电离(ESI)模式下使用，并且不能与液相色谱系统接口，这使得分析化合物混合物变得非常困难。在过去两年中，该仪器出现了一些严重的机械和操作问题，不再起作用。修复这个仪器是不可能的，因为这个旧型号几年前就被Thermo认为过时了，他们不再维修这个仪器，更换的部件很难或不可能找到。校园里没有其他低分辨率质谱仪可用于常规分析。该仪器的消亡导致申请者和威斯康星大学其他研究人员的研究计划严重延误，他们的研究计划依赖于随时可以获得低分辨率质谱仪。我们正在申请资金购买一台新的Thermo LTQ-XL质谱仪。除了可靠和可用，它还将允许分析更广泛的化合物，因为它将具有ESI和大气压化学电离能力。它还将与一个液体色谱系统相连接，该系统将使化合物混合物能够被分离和浓缩，从而可以单独分析混合物的每一种成分。所要求的Thermo LTQ-XL仪器是申请者实现他们的研究目标和目的以及培训HQP的基本工具，这是学术界和工业界最重要的分析技术之一。

This application is for funds to purchase a low-resolution mass spectrometer. A mass spectrometer is an important research instrument that is used to determine the molecular weight of a molecule. One of the mass spectrometers housed in The University of Waterloo Mass Spectrometry Facility (UWMSF) is a low-resolution instrument called a Thermo LTQ. It was used by graduate students and post-doctoral fellows for rapid routine measurements when high-resolution mass spectrometry data is not required. It was also used by HQP for developing suitable analysis conditions before acquiring high-resolution MS data on the facility's more sophisticated (and fragile and expensive) high-resolution mass spectrometers. This pre-screening of conditions is very important as it increases the life span and decreases the repair and maintenance costs of the more expensive, high-resolution instruments. The Thermo LTQ instrument was obtained used from The Princess Margaret Hospital of Toronto in 2013 and is 16 years old. It could only be used in electrospray ionization (ESI) mode and was not interfaced to a liquid chromatography system which made analyzing compound mixtures very difficult. This instrument has experienced a number of serious mechanical and operational issues over the past two years and is no longer functioning. Repairing this instrument is not possible as this old model was deemed obsolete several years ago by Thermo who no longer service the instrument and replacement parts are difficult or impossible to source. There are no other low resolution mass spectrometers on campus that are available for routine analysis. The demise of this instrument is causing significant delays in the research programs of the applicants and other researchers at UW whose research programs depend upon ready access to low-resolution mass spectrometry. We are requesting funds to purchase a new Thermo LTQ-XL mass spectrometer. Besides being reliable and serviceable, it will also allow for a much wider range of compounds to be analyzed as it would have both ESI and atmospheric pressure chemical ionization capabilities. It will also be interfaced with a liquid chromatography system which will enable mixtures of compounds to be separated and concentrated and so each component of the mixture can be individually analyzed. The requested Thermo LTQ-XL instrument is an essential tool for the applicants to realize their research goals and objectives and to train HQP in one of the most important analytical techniques in academia and industry.