Measuring active muscle oxygenation and blood flow non-invasively during dynamic physical activity

该提案要求资金购买一台耗氧代谢率光学监测仪（MetaOx），以探索个人如何在体力活动期间将氧气输送与氧气需求相匹配，以确定和理解氧气输送途径中存在个性化策略和结果的方式和原因。所需设备：MetaOx是一种无创监测仪，结合了主动组织氧合（频域近红外光谱）和血流（扩散相关光谱）的测量。该监测仪将是加拿大运动生理学实验室的第一台，也是全国的第二台。它将用于：该监测器将成为我的研究计划的广泛支柱，并将用于一种新的实验方法，重点关注心脏和血管如何使氧气输送与氧气需求相匹配的个体差异。当人们进行步行、跑步或骑自行车等体力活动时，氧气输送必须与肌肉的氧气需求相匹配。心脏和血管之间的协调反应有助于促进最佳匹配，并使这些日常生活活动（ADL）得到良好的耐受。启用的活动：该监护仪将允许在反映ADL的动态运动（包括跑步机行走、跑步或骑自行车）期间对主动肌肉氧合和血流量进行无创测量，同时允许将知识从实验室直接转化为现实世界。目前，为了评估主动肌肉氧输送，我的实验室通过动脉回声和多普勒超声无创地测量肌肉血流量，而静脉血样提供了主动肌肉氧提取的测量。然而，使用超声波需要等长收缩，其中关节和肢体不移动。这并不能反映日常生活活动中常见的动态肌肉运动的真实状态，而且还评估了“大量”肢体血流量，而不是专门评估活动肌肉。有了这个监测器，我的实验室将能够解构从口腔到肌肉的氧气输送途径，同时通过关注独特的个体反应，实现科学方法的范式转变。影响和意义：确定如何以及为什么个性化的策略和结果存在于氧气输送途径是很重要的，并适用于，加拿大人口在广大和代表一个关键的缺失环节，了解控制血管扩张，个人能力，进行体力活动，以及易受劳累不耐受。所要求的设备的应用将创造必要的基础知识，为有针对性的、个性化的或“精确”的干预战略提供信息，类似于个性化医疗的新兴领域，以改善所有加拿大人的生活，从高性能运动到康复环境。

This proposal requests funds to purchase an Optical Monitor of Metabolic Rate of Oxygen Consumption (MetaOx) to explore how individuals match oxygen delivery to oxygen demand during physical activity in order to identify and understand how and why individualized strategies and outcomes exist in the oxygen delivery pathway. Requested equipment: The MetaOx is a non-invasive monitor combining measures of active tissue oxygenation (frequency-domain near infrared spectroscopy) and blood flow (diffuse correlation spectroscopy). This monitor will be the first within an exercise physiology laboratory within Canada and the second within the nation. What it will be used for: This monitor will form a broad backbone of my research program and will be utilized within a novel experimental approach focusing on differences between individuals in how the heart and blood vessels match oxygen delivery to oxygen demand. When people perform physical activities like walking, running or cycling, oxygen delivery must be matched to the muscle's oxygen demand. Coordinated responses between the heart and blood vessels help facilitate an optimal matching and allow these activities of daily living (ADL) to be well-tolerated. Enabled activities: This monitor will allow for non-invasive measurement of active muscle oxygenation and blood flow during dynamic movements reflective of ADL including treadmill walking, running, or cycling while allowing direct knowledge translation from the laboratory to the real-world. Presently, to assess active muscle oxygen delivery, my laboratory measures muscle blood flow non-invasively by arterial echo and Doppler ultrasound while venous blood samples provide a measure of oxygen extraction by the active muscle. However, the use of ultrasound requires isometric contraction in which the joint and limb do not move. This does not reflect the true state of dynamic muscular movements common to ADL and also assesses `bulk' limb blood flow as opposed to active muscle specifically. With this monitor, my laboratory will be able to deconstruct the oxygen delivery pathway from the mouth to the muscle, while enabling a paradigm shift in the scientific approach by focusing on unique individual responses. Impact and significance: Identifying how and why individualized strategies and outcomes exist in the oxygen delivery pathway is important for, and applicable to, the Canadian population at large and represents a critical missing link in understanding the control of blood vessel dilation, the individual capacity to perform physical activity, and the vulnerability to exertional intolerance. Application of the requested equipment will create the foundational knowledge required to inform strategies of targeted, personalized or `precision' interventions, similar to the emerging field of personalized medicine, to improve the lives of all Canadians ranging from high performance exercise through to rehabilitation settings.