Sex differences in alcohol dehydrogenase levels (ADH) and blood ethanol concentration (BEC) in Japanese quail.
Sex differences in alcohol dehydrogenase levels (ADH) and blood ethanol concentration (BEC) in Japanese quail.
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DOI：
10.1016/j.psj.2022.101790
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发表时间：
2022-05
期刊：
影响因子：
4.4
通讯作者：
Akins CK
中科院分区：
文献类型：
--
作者：
Eaton SE;Jagielo-Miller JE;Prendergast MA;Akins CK
乙醇是最广泛使用和滥用的药物之一。乙醇消耗后，乙醇从小肠进入血液，在那里它分布到外周组织。在血液中，乙醇被肝脏从系统中清除。乙醇的主要代谢利用乙醇脱氢酶（ADH）。在哺乳动物中，雌性动物的肝脏样品中ADH活性似乎高于雄性动物。第一个实验的目的是分析乙醇给药12天后ADH水平的性别差异（即，水或2g/kg）。在最后一次每日乙醇处理后，将鹌鹑安乐死，提取其肝脏，并分析肝脏匀浆样品中的ADH。结果表明，雌鹌鹑比雄鹌鹑具有更高的ADH水平，更重的肝脏，和更大的肝体重比。在第二个实验中，我们的目的是开发一个血液乙醇浓度（BEC）的男性和女性鹌鹑。鹌鹑灌胃乙醇0.75或2 g/kg，分别于灌胃后0.5、1、2、4、6、8、12、24 h采血。使用Analox分析血液乙醇浓度。我们发现，鹌鹑有一个相当迅速的增加BEC其次是一个稳定和缓慢的消失乙醇从血液样本。雌鹌鹑乙醇浓度的峰值和曲线下面积均低于雄鹌鹑。目前的研究表明，雌性鹌鹑体内较高的ADH水平可能是导致乙醇代谢增加的原因。一般来说，鹌鹑似乎比啮齿动物更慢地消除乙醇。因此，作为一个模型，它们可以允许一个延长的窗口，以研究乙醇的影响。
Ethanol is one of the most widely used and abused drugs. Following ethanol consumption, ethanol enters the bloodstream from the small intestine where it gets distributed to peripheral tissues. In the bloodstream, ethanol is cleared from the system by the liver. The primary metabolism of ethanol uses alcohol dehydrogenase (ADH). In mammals, females appear to have higher ADH activity in liver samples than males. The purpose of the first experiment was to analyze sex differences in ADH levels following 12 d of ethanol administration (i.e., water or 2 g/kg) in male and female quail. Following the last daily treatment of ethanol, quail were euthanized, their livers were extracted, and ADH was analyzed in liver homogenate samples. Results showed that female quail had higher ADH levels, heavier livers, and a greater liver to body weight ratio than male quail. In a second experiment, we aimed to develop a blood ethanol concentration (BEC) profile for both male and female quail. Quail were administered 0.75 or 2 g/kg of ethanol and blood was collected at 0.5, 1, 2, 4, 6, 8, 12, 24 h after gavage administration. Blood ethanol concentration was analyzed using an Analox. We found that quail had a fairly rapid increase in BECs followed by a steady and slow disappearance of ethanol from the blood samples. Female quail had a lower peak of ethanol concentration and a smaller area under the curve (AUC) than male quail. The current research suggests that higher ADH levels in female quail may be responsible for increased metabolism of ethanol. In general, quail appear to eliminate ethanol more slowly than rodents. Thus, as a model, they may allow for a prolonged window with which to investigate the effects of ethanol.
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DOI：
10.1007/7854_2011_135
发表时间：
2013-01-01
期刊：
BEHAVIORAL NEUROBIOLOGY OF ALCOHOL ADDICTION
影响因子：
--
作者：
Hendler, Reuben A.;Ramchandani, Vijay A.;Hommer, Daniel W.
通讯作者：
Hommer, Daniel W.
影响因子：
4.2
作者：
DAJANI, RM;ORTEN, JM;DANIELSKI, J
通讯作者：
DANIELSKI, J
DOI：
10.1111/j.1530-0277.1993.tb00751.x
发表时间：
1993-04-01
影响因子：
3.2
作者：
MARTIN, CS;MOSS, HB
通讯作者：
MOSS, HB
影响因子：
2.3
作者：
DESROCHES, D;OREVILLO, C;VERINA, D
通讯作者：
VERINA, D
DOI：
10.1111/j.1530-0277.1998.tb05897.x
发表时间：
1998-12-01
影响因子：
3.2
作者：
Holdstock, L;de Wit, H
通讯作者：
de Wit, H