Vitamin A Deficiency in the Early-Life Periods Alters a Diversity of the Colonic Mucosal Microbiota in Rats.
Vitamin A Deficiency in the Early-Life Periods Alters a Diversity of the Colonic Mucosal Microbiota in Rats.
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生命早期维生素 A 缺乏会改变大鼠结肠粘膜微生物群的多样性。
DOI：
10.3389/fnut.2020.580780
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发表时间：
2020
影响因子：
5
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Chen B;Liu S;Feng D;Xiao L;Yang T;Li T;Sun W;Chen J
维生素A缺乏症（VAD）仍然是一个全球性的公共卫生问题，影响孕妇和儿童。生命早期微生物群是调节宿主免疫和代谢发育的潜在有效干预目标。本文研究了不同生命阶段VAD对青春期大鼠结肠粘膜微生物群结构的影响。结果表明，VA正常（货车）大鼠母体血清视黄醇浓度> ~1.05 μmol/L，VAD大鼠母体血清视黄醇浓度< 0.7 μmol/L，货车组仔鼠血清视黄醇浓度> 0.7 μ mol/L，VAD组仔鼠血清视黄醇浓度<0.5 μmol/L。与从胚胎期开始持续使用货车的后代（A组）相比，所有其余组均表现出厚壁菌门/拟杆菌门丰度的增加比率。宏基因组分析（LEfSe）和Metastats的属丰度差异特征分析表明，胚胎期持续VAD的子代（B组）中，黄曲霉菌和嗜冷杆菌增加，断奶后VAD的子代（C组）中，双歧杆菌减少，葡萄球菌增加;妊娠期VAD子代（E组）丙酸杆菌和肠杆菌明显增多; B组和VAD子代（D组）在妊娠期和哺乳期苍白杆菌增多。粪杆菌丰度与血清视黄醇水平呈显著正相关，葡萄球菌丰度与血清视黄醇水平呈显著负相关。不同生命时期的VAD可以改变大鼠的肠道微生物组，但生命早期（特别是妊娠期和/或哺乳期）的VAD会导致青春期大鼠结肠粘膜微生物群的多样性以及硬壁菌门和拟杆菌门之间的比例失衡。生命早期可能成为VA干预改善VA缺乏所致肠道菌群的时间窗口，但具体机制需要更深入的研究。
Vitamin A deficiency (VAD) remains a public health issue worldwide, affecting pregnant women and children. The early-life microbiota is a potentially effective intervention target for modulating immune and metabolic development of the host. This paper investigates the effects of VAD during different life periods on the structure of the colonic mucosa microbiota in adolescent rats. The results showed that the concentrations of serum retinol were > ~1.05 μmol/L in maternal VA normal (VAN)rats and < 0.7 μmol/L in maternal VAD rats, while the serum retinol levels were higher than 0.7 μmol/L in the pups of the VAN group and below 0.5 μmol/L in the pups of the VAD group. Compared to the offspring persistent with VAN from embryonic stage (group A), all the remaining groups exhibited an increased ratio of Firmicutes/Bacteroidetes abundance. A metagenome analysis (LEfSe) and a differentially abundant features approach using Metastats for genus abundances revealed that Diaphorobacter and Psychrobacter were increased in the offspring persistent with VAD from embryonic stage (group B);Bifidobacterium was decreased and Staphylococcus was increased in the offspring with VAD after weaning (group C); Propionibacterium and Enterobacter were increased significantly in the offspring with VAD during gestation(group E); and Ochrobactrum was increased in group B and the offspring with VAD during gestation and lactation(group D). Faecalibacterium abundance was significantly and positively related to serum retinol levels, while that of Staphylococcus was significantly and negatively correlated with serum retinol levels. VAD in different life periods can alter the gut microbiome in rats, but VAD in the early-life periods (especially gestation and/or lactation) leads to a diversity of the colonic mucosal microbiota in adolescent rats as well as an imbalance of the ratio between Firmicutes and Bacteroidetes. The early-life period may become a time window of VA intervention to improve intestinal microbiota caused by VA deficiency, but the specific mechanism requires more in-depth research.
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影响因子：
10.6
作者：
Carlson AL;Xia K;Azcarate-Peril MA;Goldman BD;Ahn M;Styner MA;Thompson AL;Geng X;Gilmore JH;Knickmeyer RC
通讯作者：
Knickmeyer RC
影响因子：
4.6
作者：
Lee H;Ko G
通讯作者：
Ko G
影响因子：
4.2
作者：
Galley JD;Nelson MC;Yu Z;Dowd SE;Walter J;Kumar PS;Lyte M;Bailey MT
通讯作者：
Bailey MT
影响因子：
4.4
作者：
Chen, Ke;Zhang, Xuan;Liu, You-xue
通讯作者：
Liu, You-xue
影响因子：
5.9
作者：
Cañete A;Cano E;Muñoz-Chápuli R;Carmona R
通讯作者：
Carmona R