Ovariectomy results in variable changes in nociception, mood and depression in adult female rats.
Ovariectomy results in variable changes in nociception, mood and depression in adult female rats.
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DOI：
10.1371/journal.pone.0094312
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhang YQ
中科院分区：
文献类型：
--
作者：
Li LH;Wang ZC;Yu J;Zhang YQ
绝经期卵巢激素的下降可能会影响躯体感觉、认知和情感的处理。本研究探讨了激素耗竭是否会改变卵巢切除术（OVX）后实验大鼠的伤害性，抑郁样和学习行为，这是一种常见的方法来耗尽动物的性腺激素。OVX大鼠在OVX后2周出现近尾和远尾热痛觉过敏，并持续7周。在OVX后5周也发生了强烈的机械异常性疼痛。在OVX后第5周，稀释福尔马林（5%）诱导的伤害性反应（如抬起和舔或咬）在第二阶段显着增加相比，完整的和假OVX的女性。然而，坐骨神经引起的机械性异常性疼痛的慢性压迫性损伤（CCI）与激素状态（例如OVX和卵巢完整）没有差异。使用福尔马林诱导的条件性位置回避（F-CPA），这被认为是反映了疼痛相关的负面情绪，我们进一步发现，OVX显着衰减F-CPA分数，但没有改变电击足诱导的CPA（S-CPA）。在旷场和强迫游泳实验中，OVX大鼠抑郁样行为增加。术后5周，OVX大鼠的Morris水迷宫任务未检测到空间操作能力受损。雌激素替代可恢复卵巢切除诱导的伤害性超敏反应和抑郁样行为。这是首次以统一和标准的方式研究卵巢切除对成年雌性大鼠伤害性感知、负性情绪、抑郁样行为和空间学习的影响。
Decline in the ovarian hormones with menopause may influence somatosensory, cognitive, and affective processing. The present study investigated whether hormonal depletion alters the nociceptive, depressive-like and learning behaviors in experimental rats after ovariectomy (OVX), a common method to deplete animals of their gonadal hormones. OVX rats developed thermal hyperalgesia in proximal and distal tail that was established 2 weeks after OVX and lasted the 7 weeks of the experiment. A robust mechanical allodynia was also occurred at 5 weeks after OVX. In the 5th week after OVX, dilute formalin (5%)-induced nociceptive responses (such as elevating and licking or biting) during the second phase were significantly increased as compared to intact and sham-OVX females. However, chronic constriction injury (CCI) of the sciatic nerve-induced mechanical allodynia did not differ as hormonal status (e.g. OVX and ovarian intact). Using formalin-induced conditioned place avoidance (F-CPA), which is believed to reflect the pain-related negative emotion, we further found that OVX significantly attenuated F-CPA scores but did not alter electric foot-shock-induced CPA (S-CPA). In the open field and forced swimming test, there was an increase in depressive-like behaviors in OVX rats. There was no detectable impairment of spatial performance by Morris water maze task in OVX rats up to 5 weeks after surgery. Estrogen replacement retrieved OVX-induced nociceptive hypersensitivity and depressive-like behaviors. This is the first study to investigate the impacts of ovarian removal on nociceptive perception, negative emotion, depressive-like behaviors and spatial learning in adult female rats in a uniform and standard way.
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影响因子：
2.9
作者：
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