Biophysical characteristics reveal neural stem cell differentiation potential.
Biophysical characteristics reveal neural stem cell differentiation potential.
复制标题
DOI：
10.1371/journal.pone.0025458
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Flanagan LA
中科院分区：
文献类型：
--
作者：
Labeed FH;Lu J;Mulhall HJ;Marchenko SA;Hoettges KF;Estrada LC;Lee AP;Hughes MP;Flanagan LA
区分人类神经干/祖细胞（huNSPC）群体，将主要产生神经元，从那些产生神经胶质细胞，目前阻碍了缺乏足够的细胞类型特异性的表面标志物预测的命运潜力。这限制了谱系偏向祖细胞的研究及其作为治疗剂的潜在用途。一个活细胞的生物物理和标记的命运潜力的措施将解决这个问题，消除了特定的细胞表面标记的需要。我们使用介电电泳（DEP）来分析生物物理，特别是电生理，皮质的人类和小鼠的NSPCs的分化潜力不同的属性。我们的数据表明，电生理特性膜电容与NSPCs的神经原性电位呈负相关。此外，随着huNSPCs不断传代，它们减少神经元生成并增加膜电容，证实该参数动态预测神经原性潜力并与之负相关。相比之下，NSPCs之间的膜电导差异并不总是与细胞产生神经元的能力相关。DEP交叉频率是DEP中细胞行为的定量测量，与NSPC的神经元生成直接相关，表明分离偏向特定分化细胞命运的干细胞的潜在机制。我们在这里表明，整个细胞膜电容，但不是膜电导，反映和预测的神经原性潜力的人类和小鼠的NSPCs。因此，干细胞的生物物理特性提供了一个全新的和定量的干细胞命运的潜力和无标记的手段，以确定神经元或胶质细胞偏向的祖细胞。
Distinguishing human neural stem/progenitor cell (huNSPC) populations that will predominantly generate neurons from those that produce glia is currently hampered by a lack of sufficient cell type-specific surface markers predictive of fate potential. This limits investigation of lineage-biased progenitors and their potential use as therapeutic agents. A live-cell biophysical and label-free measure of fate potential would solve this problem by obviating the need for specific cell surface markers. We used dielectrophoresis (DEP) to analyze the biophysical, specifically electrophysiological, properties of cortical human and mouse NSPCs that vary in differentiation potential. Our data demonstrate that the electrophysiological property membrane capacitance inversely correlates with the neurogenic potential of NSPCs. Furthermore, as huNSPCs are continually passaged they decrease neuron generation and increase membrane capacitance, confirming that this parameter dynamically predicts and negatively correlates with neurogenic potential. In contrast, differences in membrane conductance between NSPCs do not consistently correlate with the ability of the cells to generate neurons. DEP crossover frequency, which is a quantitative measure of cell behavior in DEP, directly correlates with neuron generation of NSPCs, indicating a potential mechanism to separate stem cells biased to particular differentiated cell fates. We show here that whole cell membrane capacitance, but not membrane conductance, reflects and predicts the neurogenic potential of human and mouse NSPCs. Stem cell biophysical characteristics therefore provide a completely novel and quantitative measure of stem cell fate potential and a label-free means to identify neuron- or glial-biased progenitors.
登录
查看更多内容
影响因子：
16.2
作者：
MayerProschel, M;Kalyani, AJ;Rao, MS
通讯作者：
Rao, MS
影响因子：
3.8
作者：
Altomare, L;Borgatti, M;Gambari, R
通讯作者：
Gambari, R
DOI：
10.1073/pnas.92.3.860
发表时间：
1995-01-31
影响因子：
11.1
作者：
BECKER, FF;WANG, XB;GASCOYNE, PRC
通讯作者：
GASCOYNE, PRC
影响因子：
3.5
作者：
Broche, LM;Labeed, FH;Hughes, MP
通讯作者：
Hughes, MP
影响因子：
4.4
作者：
Gascoyne, PRC;Wang, XB;Becker, FF
通讯作者：
Becker, FF