Emulsion-free chitosan-genipin microgels for growth plate cartilage regeneration.
Emulsion-free chitosan-genipin microgels for growth plate cartilage regeneration.
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DOI：
10.1177/0885328221999894
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发表时间：
2021-08
影响因子：
2.9
通讯作者：
Krebs M
中科院分区：
文献类型：
--
作者：
Erickson C;Stager M;Riederer M;Payne KA;Krebs M
生长板是儿童长骨末端附近的软骨组织，负责骨骼生长。对生长板的损伤会导致横跨生长板的“骨条”的形成，从而导致儿童骨骼生长异常。生物材料，如壳聚糖微凝胶，可能是一种潜在的治疗生长板损伤的方法，因为它们具有软骨形成特性，可以通过负载生物制剂来增强。它们通常是通过乳化法制造的，这种方法涉及到具有细胞毒性的溶剂漂洗。在这里，我们提出了一种高通量、无细胞毒性、非乳化的方法来制备壳聚糖-京尼平微凝胶。壳聚糖与京尼平交联形成水凝胶网络，然后通过注射器过滤器使用不同孔径的网目来制备各种尺寸的微凝胶颗粒。然后将微凝胶负载趋化因子和生长因子，并进行体外释放研究。为了评估微凝胶用于生长板软骨再生的适用性，将其注射到大鼠生长板损伤中。它们导致软骨修复组织增加，并在体内28天内完全降解。这项工作证明了壳聚糖微凝胶可以在不需要溶剂清洗的情况下制备，并展示了它们在治疗生长板损伤方面的潜力。
The growth plate is a cartilage tissue near the ends of children’s long bones and is responsible for bone growth. Injury to the growth plate can result in the formation of a ‘bony bar’ which can span the growth plate and result in bone growth abnormalities in children. Biomaterials such as chitosan microgels could be a potential treatment for growth plate injuries due to their chondrogenic properties, which can be enhanced through loading with biologics. They are commonly fabricated via an emulsion method, which involves solvent rinses that are cytotoxic. Here, we present a high throughput, non-cytotoxic, non-emulsion-based method to fabricate chitosan-genipin microgels. Chitosan was crosslinked with genipin to form a hydrogel network, and then pressed through a syringe filter using mesh with various pore sizes to produce a range of microgel particle sizes. The microgels were then loaded with chemokines and growth factors and their release was studied in vitro. To assess the applicability of the microgels to be used for growth plate cartilage regeneration, they were injected into a rat growth plate injury. They led to increased cartilage repair tissue and were fully degraded by 28 days in vivo. This work demonstrates that chitosan microgels can be fabricated without solvent rinses and demonstrates their potential for the treatment of growth plate injuries.
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DOI：
10.1016/j.ijbiomac.2017.03.142
发表时间：
2017-11-01
影响因子：
8.2
作者：
Neethu, Mohan;Mohanan, P., V;Prabha, Nair
通讯作者：
Prabha, Nair
DOI：
10.1002/jbm.b.34580
发表时间：
2020-02-08
影响因子：
3.4
作者：
Erickson, Christopher B.;Newsom, Jake P.;Payne, Karin A.
通讯作者：
Payne, Karin A.
影响因子：
11.2
作者：
Riederer MS;Requist BD;Payne KA;Way JD;Krebs MD
通讯作者：
Krebs MD
影响因子：
1
作者：
Comblain, Fanny;Rocasalbas, Guillem;Henrotin, Yves
通讯作者：
Henrotin, Yves
影响因子：
1.2
作者：
Erickson, Christopher B.;Shaw, Nichole;Payne, Karin A.
通讯作者：
Payne, Karin A.