The effects of processing methods upon mechanical and biologic properties of porcine dermal extracellular matrix scaffolds.
The effects of processing methods upon mechanical and biologic properties of porcine dermal extracellular matrix scaffolds.
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DOI：
10.1016/j.biomaterials.2010.07.083
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发表时间：
2010-11
期刊：
影响因子：
14
通讯作者：
Badylak, Stephen F.
中科院分区：
文献类型：
--
作者：
Reing, Janet E.;Brown, Bryan N.;Daly, Kerry A.;Freund, John M.;Gilbert, Thomas W.;Hsiong, Susan X.;Huber, Alexander;Kullas, Karen E.;Tottey, Stephen;Wolf, Matthew T.;Badylak, Stephen F.
关键词：
来自不同物种和组织的生物材料通常被用作组织重建的外科网状物或支架。细胞外基质(ECM)是组成每个组织和器官的细胞的分泌产物，因此为特定的再生医学应用提供了独特的生物材料。在组织处理过程中对ECM超微结构和内容物的最小破坏通常是可取的。本研究的目的是系统地评价常用的组织加工步骤对猪真皮ECM支架组成、力学性能和细胞相容性的影响。评估的处理步骤包括石灰和热水卫生、胰酶/十二烷基硫酸钠/TritonX-100脱细胞和胰酶/TritonX-100脱细胞。石灰降低了细胞外基质的生长因子和糖胺多糖含量、机械强度和支持细胞生长的能力(p≤均0.05)。热水卫生处理仅降低细胞外基质中生长因子的含量(p≤0.05)。胰酶/十二烷基硫酸钠/TritonX-100脱细胞处理可降低细胞外基质的生长因子含量和支持细胞生长的能力(p≤均为0.05)。胰酶/TritonX-100脱细胞处理也降低了细胞外基质中生长因子的含量，但增强了细胞外基质支持体外细胞生长的能力(p≤均为0.05)。我们的结论是，本研究中评估的加工步骤会影响所生成的猪真皮ECM的含量、机械强度和/或细胞相容性，因此必须谨慎选择适当的加工步骤，以保持ECM在生物支架中的有益效果。
Biologic materials from various species and tissues are commonly used as surgical meshes or scaffolds for tissue reconstruction. Extracellular matrix (ECM) represents the secreted product of the cells comprising each tissue and organ, and therefore provides a unique biologic material for selected regenerative medicine applications. Minimal disruption of ECM ultrastructure and content during tissue processing is typically desirable. The objective of this study was to systematically evaluate effects of commonly used tissue processing steps upon porcine dermal ECM scaffold composition, mechanical properties, and cytocompatibility. Processing steps evaluated included liming and hot water sanitation, trypsin/SDS/TritonX-100 decellularization, and trypsin/TritonX-100 decellularization. Liming decreased the growth factor and glycosaminoglycan content, the mechanical strength, and the ability of the ECM to support in vitro cell growth (p ≤ 0.05 for all). Hot water sanitation treatment decreased only the growth factor content of the ECM (p ≤ 0.05). Trypsin/SDS/TritonX-100 decellularization decreased the growth factor content and the ability of the ECM to support in vitro cell growth (p ≤ 0.05 for both). Trypsin/TritonX-100 decellularization also decreased the growth factor content of the ECM but increased the ability of the ECM to support in vitro cell growth (p ≤ 0.05 for both). We conclude that processing steps evaluated in the present study affect content, mechanical strength, and/or cytocompatibility of the resultant porcine dermal ECM, and therefore care must be taken in choosing appropriate processing steps to maintain the beneficial effects of ECM in biologic scaffolds.
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影响因子：
14
作者：
Brown, Bryan N.;Barnes, Christopher A.;Kasick, Rena T.;Michel, Roger;Gilbert, Thomas W.;Beer-Stolz, Donna;Castner, David G.;Ratner, BuddyD.;Badylak, Stephen F.
通讯作者：
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