Solanine induces mitochondria-mediated apoptosis in human pancreatic cancer cells.
Solanine induces mitochondria-mediated apoptosis in human pancreatic cancer cells.
复制标题
茄碱诱导人胰腺癌细胞线粒体介导的细胞凋亡
DOI：
10.1155/2014/805926
复制
发表时间：
2014
影响因子：
--
通讯作者：
Zhou M
中科院分区：
文献类型：
--
作者：
Sun H;Lv C;Yang L;Wang Y;Zhang Q;Yu S;Kong H;Wang M;Xie J;Zhang C;Zhou M
甾体生物碱已被认为是潜在的抗癌化合物。然而，类固醇生物碱如何抑制肿瘤生长的潜在机制在很大程度上是未知的。在此，我们报道了龙葵碱，一种甾体生物碱的物质，在体外和体内对胰腺癌细胞生长的抑制具有积极的作用。在胰腺癌细胞和nu/nu裸鼠模型中，我们发现龙葵碱通过caspase-3依赖的线粒体凋亡抑制癌细胞生长。在机械上，龙葵碱通过下调Bcl-2/Bax比率促进线粒体膜通透性转换孔（MPTP）的开放;此后，细胞色素c和Smac从线粒体释放到细胞质中以将半胱天冬酶-3酶原加工成活化形式。此外，我们发现在龙葵碱处理的细胞中，肿瘤转移相关蛋白MMP-2和MMP-9的表达也降低。因此，我们的研究结果表明龙葵碱是一种有效的化合物，用于治疗胰腺癌。
Steroid alkaloids have been suggested as potential anticancer compounds. However, the underlying mechanisms of how steroid alkaloids inhibit the tumor growth are largely unknown. Here, we reported that solanine, a substance of steroid alkaloids, has a positive effect on the inhibition of pancreatic cancer cell growth in vitro and in vivo. In pancreatic cancer cells and nu/nu nude mice model, we found that solanine inhibited cancer cells growth through caspase-3 dependent mitochondrial apoptosis. Mechanically, solanine promotes the opening of mitochondrial membrane permeability transition pore (MPTP) by downregulating the Bcl-2/Bax ratio; thereafter, Cytochrome c and Smac are released from mitochondria into cytosol to process the caspase-3 zymogen into an activated form. Moreover, we found that the expression of tumor metastasis related proteins, MMP-2 and MMP-9, was also decreased in the cells treated with solanine. Therefore, our results suggested that solanine was an effective compound for the treatment of pancreatic cancer.
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影响因子：
6.1
作者：
Friedman, M;Lee, KR;Kozukue, N
通讯作者：
Kozukue, N
影响因子：
5.4
作者：
Ji, Y. B.;Gao, S. Y.;Zou, X.
通讯作者：
Zou, X.
DOI：
10.1007/978-1-4614-1680-7_8
发表时间：
2012-01-01
期刊：
SELF AND NONSELF
影响因子：
--
作者：
Munoz-Pinedo, Cristina
通讯作者：
Munoz-Pinedo, Cristina
影响因子：
4.3
作者：
Shi-Yong Gao;Qiu-Juan Wang;Yu-Bin Ji
通讯作者：
Yu-Bin Ji
影响因子：
56.9
作者：
Spierings, D;McStay, G;Green, DR
通讯作者：
Green, DR