Transposon mutagenesis of the plant-associated Bacillus amyloliquefaciens ssp. plantarum FZB42 revealed that the nfrA and RBAM17410 genes are involved in plant-microbe-interactions.
Transposon mutagenesis of the plant-associated Bacillus amyloliquefaciens ssp. plantarum FZB42 revealed that the nfrA and RBAM17410 genes are involved in plant-microbe-interactions.
复制标题
DOI：
10.1371/journal.pone.0098267
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Borriss R
中科院分区：
文献类型：
--
作者：
Budiharjo A;Chowdhury SP;Dietel K;Beator B;Dolgova O;Fan B;Bleiss W;Ziegler J;Schmid M;Hartmann A;Borriss R
淀粉芽孢杆菌植物亚种plantarum FZB 42代表了革兰氏阳性植物生长促进和生防细菌的原型。在这项研究中，我们应用转座子诱变产生转座子库，这是筛选基因参与多细胞行为和生物膜形成的根作为一个先决条件的植物生长促进活性。转座子插入位点通过拯救克隆随后DNA测序来确定。就像B。枯草芽孢杆菌的全局转录调节因子DegU被鉴定为集群和生物膜形成所必需的基因的激活剂，并且发现FZB 42的DegU突变体在有效的根定殖中受损。直接筛选3，000个转座子插入突变体以促进植物生长，揭示了nfrA和RBAM_017140的基因产物对于FZB 42对植物施加的有益作用是必需的。我们分析了GFP标记的野生型和转座子突变体的生菜根的殖民化，使用共聚焦激光扫描显微镜的性能。虽然野生型菌株大量定殖根表面，nfrA突变体没有定殖莴苣根，但它在实验室培养物中的生长、生物膜形成和琼脂平板上的群集运动性没有受损。RBAM 17410基因，仅存在于B的少数成员中。枯草杆菌种复合体，直接参与植物生长促进。没有一个突变株在产生植物生长激素生长素方面受到影响。我们推测nfrA基因产物对于克服植物对细菌根定殖的反应所引起的胁迫是必不可少的。
Bacillus amyloliquefaciens ssp. plantarum FZB42 represents the prototype of Gram-positive plant growth promoting and biocontrol bacteria. In this study, we applied transposon mutagenesis to generate a transposon library, which was screened for genes involved in multicellular behavior and biofilm formation on roots as a prerequisite of plant growth promoting activity. Transposon insertion sites were determined by rescue-cloning followed by DNA sequencing. As in B. subtilis, the global transcriptional regulator DegU was identified as an activator of genes necessary for swarming and biofilm formation, and the DegU-mutant of FZB42 was found impaired in efficient root colonization. Direct screening of 3,000 transposon insertion mutants for plant-growth-promotion revealed the gene products of nfrA and RBAM_017140 to be essential for beneficial effects exerted by FZB42 on plants. We analyzed the performance of GFP-labeled wild-type and transposon mutants in the colonization of lettuce roots using confocal laser scanning microscopy. While the wild-type strain heavily colonized root surfaces, the nfrA mutant did not colonize lettuce roots, although it was not impaired in growth in laboratory cultures, biofilm formation and swarming motility on agar plates. The RBAM17410 gene, occurring in only a few members of the B. subtilis species complex, was directly involved in plant growth promotion. None of the mutant strains were affected in producing the plant growth hormone auxin. We hypothesize that the nfrA gene product is essential for overcoming the stress caused by plant response towards bacterial root colonization.
登录
查看更多内容
影响因子：
3.2
作者：
Branda, SS;González-Pastor, JE;Kolter, R
通讯作者：
Kolter, R
影响因子：
3.2
作者：
Calvio, Cinzia;Osera, Cecilia;Galizzi, Alessandro
通讯作者：
Galizzi, Alessandro
影响因子：
4.4
作者：
Koumoutsi, Alexandra;Chen, Xiao-Hua;Borriss, Rainer
通讯作者：
Borriss, Rainer
影响因子：
3.7
作者：
Chowdhury SP;Dietel K;Rändler M;Schmid M;Junge H;Borriss R;Hartmann A;Grosch R
通讯作者：
Grosch R
从自然环境中分离的枯草芽孢杆菌对番茄枯萎病的生物防治取决于介导生物膜形成的保守基因。
影响因子：
5.1
作者：
Chen Y;Yan F;Chai Y;Liu H;Kolter R;Losick R;Guo JH
通讯作者：
Guo JH