Propolis varnish: antimicrobial properties against cariogenic bacteria, cytotoxicity, and sustained-release profile.
Propolis varnish: antimicrobial properties against cariogenic bacteria, cytotoxicity, and sustained-release profile.
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DOI：
10.1155/2014/348647
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发表时间：
2014
影响因子：
--
通讯作者：
Santos VR
中科院分区：
文献类型：
--
作者：
De Luca MP;Franca JR;Macedo FA;Grenho L;Cortes ME;Faraco AA;Moreira AN;Santos VR
清漆是在聚合物基质和治疗剂方面不同的制剂。在牙科中，它们用于预防龋齿。在这项研究中，我们开发了一种保护漆，考虑到对致龋细菌的保护性能。向壳聚糖聚合物基质（CHV）中加入不同浓度的乙醇提取物：PV 1（5%）、PV 2（10%）和PV 3（15%）。采用琼脂扩散法对变形链球菌（SM）、血链球菌（SG）、唾液链球菌（SS）和干酪乳杆菌（LC）进行了抗菌活性研究。掺入的三种浓度的三氯甲烷在抑制所有微生物的生长方面是有效的，但在观察到的抑制区之间没有显著差异。MTT法测定细胞毒活性。资料以单因子变异数分析及Bonferroni检定进行统计分析。没有清漆是细胞毒性的，保持80%的活细胞，而CHV允许细胞增殖（120%）。通过将40 μL的每种保护漆应用于牛牙齿的颊面并保持在定期取出的乙醇/水溶液中进行缓释试验。根据“独立模型方法”，每种清漆的释放曲线不同，最长的是PV 3（8周）。清漆制剂对致龋细菌具有令人满意的抗微生物活性，并且具有低细胞毒性（<50%）。
Varnishes are preparations that differ in the polymeric matrix and therapeutical agents. In dentistry they are used to prevent caries. In this study we developed a propolis varnish, considering propolis properties against cariogenic bacteria. To a chitosan polymeric base (CHV) was added ethanolic propolis extract in different concentrations: PV1 (5%), PV2 (10%), and PV3 (15%). Antimicrobial activity was carried out against Streptococcus mutans (SM), Streptococcus sanguinis (SG), Streptococcus salivarius (SS), and Lactobacillus casei (LC) through agar diffusion method. The three propolis concentrations incorporated were effective in inhibiting the growth of all microorganisms, but without significant difference between the zones of inhibition observed. Cytotoxicity assay was done by MTT method. Data were analyzed by one-way ANOVA and Bonferroni test. None of the varnishes were cytotoxic, keeping 80% of viable cells, while CHV allowed cellular proliferation (120%). Sustained-release test was carried out by applying 40 μL of each varnish in the buccal surface of bovine teeth and kept in an ethanol/water solution removed in regular times. According to the “independent model approach,” the release profiles were distinct from each varnish and the most prolonged was PV3 (8 weeks). Varnish formulations had satisfactory antimicrobial activity against cariogenic bacteria and have a low cytotoxicity (<50%).
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影响因子：
2.5
作者：
Ccahuana-Vásquez, Renzo Alberto;Cury, Jaime Aparecido
通讯作者：
Cury, Jaime Aparecido
DOI：
10.1590/s0101-20612006000100028
发表时间：
2006-03-01
期刊：
Food Science and Technology (Campinas)
影响因子：
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作者：
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通讯作者：
Ferro, Vicente O.
影响因子：
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作者：
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通讯作者：
Marcucci MC
DOI：
10.1155/2012/751435
发表时间：
2012
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
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作者：
Galvão LC;Furletti VF;Bersan SM;da Cunha MG;Ruiz AL;de Carvalho JE;Sartoratto A;Rehder VL;Figueira GM;Teixeira Duarte MC;Ikegaki M;de Alencar SM;Rosalen PL
通讯作者：
Rosalen PL
影响因子：
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作者：
Liberio, Silvana A.;Pereira, Antonio Luis A.;Monteiro-Neto, Valerio
通讯作者：
Monteiro-Neto, Valerio