Antimicrobial Activity of Essential Oils against Streptococcus mutans and their Antiproliferative Effects.
Antimicrobial Activity of Essential Oils against Streptococcus mutans and their Antiproliferative Effects.
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DOI：
10.1155/2012/751435
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Rosalen PL
中科院分区：
文献类型：
--
作者：
Galvão LC;Furletti VF;Bersan SM;da Cunha MG;Ruiz AL;de Carvalho JE;Sartoratto A;Rehder VL;Figueira GM;Teixeira Duarte MC;Ikegaki M;de Alencar SM;Rosalen PL
本研究旨在通过化学表征其生物活性和抗增殖活性的组分来评估精油（EO）对变形链球菌生物膜的活性。通过水蒸馏获得20个EO，并进行抗S的抗菌试验（最小抑菌浓度（MIC）和杀菌浓度（MBC））。变异UA 159.薄层色谱法和气相色谱/质谱法用于植物化学分析。根据预定标准选择EO，并使用干柱进行分级;通过MIC和MBC评估所得级分，选择作为活性级分，并针对S进行评价。变形菌生物膜使用扫描电子显微镜检查形成的生物膜。评价了所选EO及其所选活性组分对角质形成细胞和七种人肿瘤细胞系的抗增殖活性。EO及其活性组分的MIC和MBC值显示出较强的抗菌活性。化学分析主要显示萜烯的存在。筛选出的活性部位对S.变形杆菌生物膜形成（P < 0.05）不影响糖酵解pH下降，对正常细胞系角质形成细胞无活性。结果表明，EO在低浓度下就表现出活性，其活性组分对S.变形杆菌和人肿瘤细胞系。
This study aimed to evaluate the activity of essential oils (EOs) against Streptococcus mutans biofilm by chemically characterizing their fractions responsible for biological and antiproliferative activity. Twenty EO were obtained by hydrodistillation and submitted to the antimicrobial assay (minimum inhibitory (MIC) and bactericidal (MBC) concentrations) against S. mutans UA159. Thin-layer chromatography and gas chromatography/mass spectrometry were used for phytochemical analyses. EOs were selected according to predetermined criteria and fractionated using dry column; the resulting fractions were assessed by MIC and MBC, selected as active fractions, and evaluated against S. mutans biofilm. Biofilms formed were examined using scanning electron microscopy. Selected EOs and their selected active fractions were evaluated for their antiproliferative activity against keratinocytes and seven human tumor cell lines. MIC and MBC values obtained for EO and their active fractions showed strong antimicrobial activity. Chemical analyses mainly showed the presence of terpenes. The selected active fractions inhibited S. mutans biofilm formation (P < 0.05) did not affect glycolytic pH drop and were inactive against keratinocytes, normal cell line. In conclusion, EO showed activity at low concentrations, and their selected active fractions were also effective against biofilm formed by S. mutans and human tumor cell lines.
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影响因子：
4.2
作者：
Jeon JG;Rosalen PL;Falsetta ML;Koo H
通讯作者：
Koo H
影响因子：
5.4
作者：
Eloff, JN
通讯作者：
Eloff, JN
影响因子：
5.4
作者：
Cristina Teixeira Duarte, Marta;Eduardo Leme, Ewerton;Sartoratto, Adilson
通讯作者：
Sartoratto, Adilson
影响因子：
3.1
作者：
HAWSER, SP;DOUGLAS, LJ
通讯作者：
DOUGLAS, LJ
影响因子：
5.4
作者：
Fouche, G.;Cragg, G. M.;Senabe, J.
通讯作者：
Senabe, J.