Isolation and Characterization of a Lipid Transfer Protein Gene (BplLTP1) from Betula platyphylla
Isolation and Characterization of a Lipid Transfer Protein Gene (BplLTP1) from Betula platyphylla
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白桦脂质转移蛋白基因 (BplLTP1) 的分离和表征
DOI：
10.1007/s11105-013-0571-6
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发表时间：
2013-02
影响因子：
2.1
通讯作者：
刘雪梅
中科院分区：
文献类型：
--
作者：
Minxiao Guan;Ruihai Chai;Xue Kong;刘雪梅
非特异性脂质转移蛋白（nsLTPs）广泛存在于植物中，但其功能尚不完全清楚。在这里，我们从白桦中分离并表征了一个假定的nsLTP基因BplLTP1。BplLTP1 cDNA全长638 bp，包括一个363 bp的开放阅读框(GenBank登录号：GenBank；JQ409562)。推测的蛋白BplLTP1包含一个n端信号序列，具有nsltp的特征。氨基酸序列比对显示，BplLTP1与其他已知的nsltp具有高度的相似性。基于同源性模型构建了BplLTP1的三维模型。实时荧光定量pcr分析显示，BplLTP1在不同组织间的表达水平无明显差异。BplLTP1在年轻组织中的表达水平明显高于老年组织。此外，BplLTP1在雄性花序的单核小孢子发育阶段表达上调。利用3月龄培养苗进行表达分析，结果显示外源脱落酸和水杨酸上调了BplLTP1的表达，外源茉莉酸甲酯下调了BplLTP1的表达，外源黄霉素a对BplLTP1的表达无显著影响。此外，利用pET32a-BplLTP1与大肠杆菌菌株BL21构建了原核表达体系，并进行了非生物抗逆性分析。
Although nonspecific lipid transfer proteins (nsLTPs) are widely present in plants, their functions are not fully understood. Here, we isolated and characterized a putative nsLTP gene, BplLTP1, from Betula platyphylla. The full-length cDNA of BplLTP1 is 638 bp long, including a 363-bp open reading frame (GenBank accession no. JQ409562). The putative protein BplLTP1 contains an N-terminal signal sequence and possesses the characteristic features of nsLTPs. An amino acid sequence alignment revealed that BplLTP1 shares a high level of similarity with other known nsLTPs. A 3D model of BplLTP1 was also constructed based on the homology model. Quantitative real time-PCR analysis showed that there were no obvious differences in the expression levels of BplLTP1 among different tissues. BplLTP1 displayed distinctly higher expression levels in young tissues than in older tissues. Moreover, BplLTP1 was upregulated at the mononuclear microspore developmental stages in male inflorescences. Expression analysis was performed using 3-month-old cultured seedlings, and the results revealed that the expression of BplLTP1 was upregulated by exogenous abscisic acid and salicylic acid, downregulated by exogenous methyl jasmonate, and not significantly altered by exogenous gibberellin A. In addition, a prokaryotic expression system was constructed with pET32a-BplLTP1 and Escherichia coli strain BL21 and subjected to abiotic stress resistance analysis.
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影响因子：
4.9
作者：
A. Yamada;M. Sekiguchi;T. Mimura;Y. Ozeki
通讯作者：
A. Yamada;M. Sekiguchi;T. Mimura;Y. Ozeki
影响因子：
2.9
作者：
BJELLQVIST, B;HUGHES, GJ;HOCHSTRASSER, D
通讯作者：
HOCHSTRASSER, D
影响因子：
5.2
作者：
Jung, HW;Lim, CW;Hwang, BK
通讯作者：
Hwang, BK
影响因子：
4.9
作者：
Niki, T;Mitsuhara, I;Ohashi, Y
通讯作者：
Ohashi, Y
影响因子：
4.3
作者：
Guohai Wu;A. Robertson;Xunjia Liu;P. Zheng;R. Wilen;N. T. Nesbitt;L. Gusta
通讯作者：
Guohai Wu;A. Robertson;Xunjia Liu;P. Zheng;R. Wilen;N. T. Nesbitt;L. Gusta